Effects of bridge ions, DNA species, and developing temperature on flat-lying DNA monolayers
文献类型：期刊论文
| 作者 | Song YH ; Guo CL ; Sun LL ; Wei G ; Peng CY ; Wang L ; Sun YJ ; Li Z |
| 刊名 | journal of physical chemistry b
 |
| 出版日期 | 2007 |
| 卷号 | 111期号:2页码:461-468 |
| 关键词 | ATOMIC-FORCE MICROSCOPY SELF-ASSEMBLED MONOLAYERS DIVALENT COUNTERIONS LIPID-MEMBRANES MICA SURFACE ADSORPTION BINDING GROWTH ACID CONDENSATION |
| ISSN号 | 1520-6106 |
| 通讯作者 | li z |
| 中文摘要 | recently, we have successfully constructed flat-lying dna monolayers on a mica surface (j. phys. chem. b 2006, 110, 10792-10798). in this work, the effects of various factors including bridge ions, dna species, and developing temperature on the configuration of dna monolayers have been investigated by atomic force microscopy (afm) in detail. afm results show that the species of bridge ions and developing temperature play a crucial role during the formation process. for example, the divalent cation zn2+ resulted in many dna chains stuck side by side in the monolayers due to the strong interactions between it and dna's bases or the mica surface. most dna chain's conglutinations disappeared when the developing temperature was higher than 40 degrees c. cd2+ and ca2+ produced more compact dna monolayers with some obvious aggregations, especially for the dna monolayers constructed by using ca2+ as the bridge ion. co2+ produced well-ordered, flat-lying dna monolayers similar to that of mg2+. furthermore, it was found that the flat-lying dna monolayers could still form on a mica surface when plasmid dna pbr 322 and linear dna pbr 322/pst i were used as the dna source. whereas, it was hard to form dna monolayers on a (3-aminopropyl)triethoxysilane-mica surface because the strong interactions between dna and substrate prevented the lateral movement of dna molecules. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243388800020 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/14491]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Song YH,Guo CL,Sun LL,et al. Effects of bridge ions, DNA species, and developing temperature on flat-lying DNA monolayers[J]. journal of physical chemistry b,2007,111(2):461-468. |
| APA | Song YH.,Guo CL.,Sun LL.,Wei G.,Peng CY.,...&Li Z.(2007).Effects of bridge ions, DNA species, and developing temperature on flat-lying DNA monolayers.journal of physical chemistry b,111(2),461-468. |
| MLA | Song YH,et al."Effects of bridge ions, DNA species, and developing temperature on flat-lying DNA monolayers".journal of physical chemistry b 111.2(2007):461-468. |
入库方式： OAI收割
来源：长春应用化学研究所
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