Direct electrochemistry and bioelectrocatalysis of horseradish peroxidase immobilized on active carbon
文献类型：期刊论文
| 作者 | Sun DM ; Cai CX ; Li XG ; Xing W ; Lu TH |
| 刊名 | journal of electroanalytical chemistry
 |
| 出版日期 | 2004 |
| 卷号 | 566期号:2页码:415-421 |
| 关键词 | DIRECT ELECTRON-TRANSFER BIOMEMBRANE-LIKE FILMS GOLD ELECTRODE HYDROGEN-PEROXIDE INFRARED-SPECTROSCOPY SURFACTANT FILM REDOX PROTEINS MYOGLOBIN GRAPHITE HEMOGLOBIN |
| ISSN号 | 0022-0728 |
| 通讯作者 | lu th |
| 中文摘要 | for the first time horseradish peroxidase (hrp) immobilized on the surface of active carbon powder modified at the surface of a glassy carbon electrode has been shown to undergo a direct quasi-reversible electrochemical reaction. its formal potential, e-o/, is -0.363 v in phosphate buffer solution (ph 6.8) at a scan rate of 100 mv/s and is almost independent of the scan rate in the range of 50-700 mv/s. the dependence of e-o/ on the ph of the buffer solution indicated that the conversion of hrp-fe(iii)/hrp-fe(ii) is a one-electron-transfer reaction process coupled with one-proton-transfer. the experimental results also demonstrated that the immobilized hrp retained its bioelectrocatalytic activity to the reduction of h2o2. furthermore, the hrp adsorbed oil the surface of the active carbon powder can be stored at 4 degreesc for several months without any loss of the enzyme activity. the method presented for immobilizing hrp can be easily extended to immobilize and obtain the direct electrochemistry of other enzymes. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221209900021 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/15405]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun DM,Cai CX,Li XG,et al. Direct electrochemistry and bioelectrocatalysis of horseradish peroxidase immobilized on active carbon[J]. journal of electroanalytical chemistry,2004,566(2):415-421. |
| APA | Sun DM,Cai CX,Li XG,Xing W,&Lu TH.(2004).Direct electrochemistry and bioelectrocatalysis of horseradish peroxidase immobilized on active carbon.journal of electroanalytical chemistry,566(2),415-421. |
| MLA | Sun DM,et al."Direct electrochemistry and bioelectrocatalysis of horseradish peroxidase immobilized on active carbon".journal of electroanalytical chemistry 566.2(2004):415-421. |
入库方式： OAI收割
来源：长春应用化学研究所
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