High resolution mass spectrometric alveolar proteomics: Identification of surfactant protein SP-A and SP-D modifications in proteinosis and cystic fibrosis patients
文献类型：期刊论文
| 作者 | Bai Y ; Galetskiy D ; Damoc E ; Paschen C ; Liu ZQ ; Griese M ; Liu SY ; Praybylski M |
| 刊名 | proteomics
 |
| 出版日期 | 2004 |
| 卷号 | 4期号:8页码:2300-2309 |
| 关键词 | BRONCHOALVEOLAR LAVAGE FLUID ASSISTED-LASER-DESORPTION/IONIZATION HOST-DEFENSE LUNG PROTEOLYSIS GEL |
| ISSN号 | 1615-9853 |
| 通讯作者 | przybylski m |
| 中文摘要 | in the present study, one- and two-dimensional gel electrophoresis combined with high resolution fourier transform-ion cyclotron resonance mass spectrometry (ft-icr ms) have been applied as powerful approaches for the proteome analysis of surfactant proteins sp-a and sp-d, including identification of structurally modified and truncation forms, in bronchoalveolar lavage fluid from patients with cystic fibrosis, chronic bronchitis and pulmonary alveolar proteinosis. highly sensitive micro preparation techniques were developed for matrix-assisted laser desorption/ionization (maldi) ft-icr ms analysis which provided the identification of surfactant proteins at very low levels. owing to the high resolution, ft-icr ms was found to provide substantial advantages for the structural identification of surfactant proteins from complex biological matrices with high mass determination accuracy. several protein bands corresponding to sp-a and sp-d were identified by maldi-ft-icr ms after electrophoretic separation by one- and two-dimensional gel electrophoresis, and provided the identification of structural modifications (hydroxy-proline) and degradation products. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223137300013 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/15451]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai Y,Galetskiy D,Damoc E,et al. High resolution mass spectrometric alveolar proteomics: Identification of surfactant protein SP-A and SP-D modifications in proteinosis and cystic fibrosis patients[J]. proteomics,2004,4(8):2300-2309. |
| APA | Bai Y.,Galetskiy D.,Damoc E.,Paschen C.,Liu ZQ.,...&Praybylski M.(2004).High resolution mass spectrometric alveolar proteomics: Identification of surfactant protein SP-A and SP-D modifications in proteinosis and cystic fibrosis patients.proteomics,4(8),2300-2309. |
| MLA | Bai Y,et al."High resolution mass spectrometric alveolar proteomics: Identification of surfactant protein SP-A and SP-D modifications in proteinosis and cystic fibrosis patients".proteomics 4.8(2004):2300-2309. |
入库方式： OAI收割
来源：长春应用化学研究所
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