Probing the kinetics of single molecule protein folding
文献类型：期刊论文
| 作者 | Leite VBP ; Onuchic JN ; Stell G ; Wang J |
| 刊名 | biophysical journal
 |
| 出版日期 | 2004 |
| 卷号 | 87期号:6页码:3633-3641 |
| 关键词 | RANDOM ENERGY-MODEL FLUORESCENCE SPECTROSCOPY ENZYMATIC DYNAMICS TRANSITION-STATE TEMPERATURE HETEROPOLYMERS COOPERATIVITY LANDSCAPES PATHWAYS INTERMITTENCY |
| ISSN号 | 0006-3495 |
| 通讯作者 | leite vbp |
| 中文摘要 | we propose an approach to integrate the theory, simulations, and experiments in protein-folding kinetics. this is realized by measuring the mean and high-order moments of the first-passage time and its associated distribution. the full kinetics is revealed in the current theoretical framework through these measurements. in the experiments, information about the statistical properties of first-passage times can be obtained from the kinetic folding trajectories of single molecule experiments ( for example, fluorescence). theoretical/simulation and experimental approaches can be directly related. we study in particular the temperature-varying kinetics to probe the underlying structure of the folding energy landscape. at high temperatures, exponential kinetics is observed; there are multiple parallel kinetic paths leading to the native state. at intermediate temperatures, nonexponential kinetics appears, revealing the nature of the distribution of local traps on the landscape and, as a result, discrete kinetic paths emerge. at very low temperatures, exponential kinetics is again observed; the dynamics on the underlying landscape is dominated by a single barrier. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000225426700003 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://202.98.16.49/handle/322003/15687]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Leite VBP,Onuchic JN,Stell G,et al. Probing the kinetics of single molecule protein folding[J]. biophysical journal,2004,87(6):3633-3641. |
| APA | Leite VBP,Onuchic JN,Stell G,&Wang J.(2004).Probing the kinetics of single molecule protein folding.biophysical journal,87(6),3633-3641. |
| MLA | Leite VBP,et al."Probing the kinetics of single molecule protein folding".biophysical journal 87.6(2004):3633-3641. |
入库方式： OAI收割
来源：长春应用化学研究所
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