Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody-antigen reactions
文献类型：期刊论文
| 作者 | Wang MJ ; Wang LY ; Wang G ; Ji XH ; Bai YB ; Li TJ ; Gong SY ; Li JH |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2004 |
| 卷号 | 19期号:6页码:575-582 |
| 关键词 | AC-IMPEDANCE MOLECULAR RECOGNITION ELECTRON-TRANSFER MONOLAYERS FUNCTIONALIZATION KINETICS SURFACE DNA FE |
| ISSN号 | 0956-5663 |
| 通讯作者 | bai yb |
| 中文摘要 | we used colloidal an to enhance the amount of antibody immobilized on a gold electrode and ultimately monitored the interaction of antigen-antibody by impedance measurement. self-assembly of 6 nm (diameter) colloidal an onto the self-assembled monolayers (sams) of 4-aminothiophenol modified gold electrode resulted in an easier attachment of antibody. the redox reactions of [fe(cn)(6)](4-)/[fe(cn)(6)](3-) on the gold surface were blocked due to the procedures of self-assembly of 4-aminothiophenol and antibody immobilization, which were investigated by cyclic voltammetry and impedance spectroscopy. the interaction of antigen with grafted antibody recognition layers was carried out by soaking the modified electrode into a phosphate buffer at ph 7.4 with various concentrations of antigen at 37 degreesc for 30 min. the antibody recognition layers and their interactions with various concentrations of antigen could be detected by measurements of the impedance change. the results show that this method has good correlation for detection of hepatitis b virus surface antigen in the range of 0.5-200 mug/l and a detection limit of about 50 ng/l. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000188504900007 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://202.98.16.49/handle/322003/15779]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang MJ,Wang LY,Wang G,et al. Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody-antigen reactions[J]. biosensors & bioelectronics,2004,19(6):575-582. |
| APA | Wang MJ.,Wang LY.,Wang G.,Ji XH.,Bai YB.,...&Li JH.(2004).Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody-antigen reactions.biosensors & bioelectronics,19(6),575-582. |
| MLA | Wang MJ,et al."Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody-antigen reactions".biosensors & bioelectronics 19.6(2004):575-582. |
入库方式： OAI收割
来源：长春应用化学研究所
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