Novel prefractionation method can be used in proteomic analysis
文献类型：期刊论文
| 作者 | Bai HX ; Yang F ; Yang XR |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2006 |
| 卷号 | 5期号:4页码:840-845 |
| 关键词 | AQUEOUS 2-PHASE SYSTEMS PROTEIN-PURIFICATION MEMBRANE-PROTEINS EXTRACTION FRACTIONATION EQUILIBRIUM COPOLYMER |
| ISSN号 | 1535-3893 |
| 通讯作者 | yang xr |
| 中文摘要 | for the first time, a novel prefractionation method used in proteomic analysis was developed, which is performed by a novel aqueous two-phase system (natps) composed of n-butanol, (nh4)(2)so4, and water. it can separate proteomic proteins into multigroups by one-step extraction. the phase-separation conditions of n-butanol solutions were studied in the presence of commonly used inorganic salts. the natps was subsequently developed. using human serum albumin, zein, and gamma-globulin as model proteins, the separation effectiveness of the natps for protein was studied under affection factors, i.e., ph, n-butanol volume, protein, or salt concentration. the model and actual protein samples were separated by the natps and then directly used for gel electrophoresis without separating the target proteins from phase-forming reagents. it revealed that the natps could separate proteomic proteins into multigroups by one-step extraction. the natps has the advantages of rapidity, simplicity, low cost, biocompability, and high efficiency. it need not separate target proteins from the phase-forming reagents. the natps has great significance in separation and extraction of proteomic proteins, as well as in methodology. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236816100013 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/15857]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai HX,Yang F,Yang XR. Novel prefractionation method can be used in proteomic analysis[J]. journal of proteome research,2006,5(4):840-845. |
| APA | Bai HX,Yang F,&Yang XR.(2006).Novel prefractionation method can be used in proteomic analysis.journal of proteome research,5(4),840-845. |
| MLA | Bai HX,et al."Novel prefractionation method can be used in proteomic analysis".journal of proteome research 5.4(2006):840-845. |
入库方式： OAI收割
来源：长春应用化学研究所
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