Characterization of prolidase activity using capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection and application to evaluate collagen degradation in diabetes mellitus
文献类型：期刊论文
| 作者 | Yuan JP ; Li T ; Yin XB ; Guo L ; Jiang XZ ; Jin WR ; Yang XR ; Wang EK |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2006 |
| 卷号 | 78期号:9页码:2934-2938 |
| 关键词 | CHEMILUMINESCENCE DETECTION IV COLLAGEN COMPLICATIONS ERYTHROCYTES NEPHROPATHY MARKER |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang ek |
| 中文摘要 | a new method for prolidase (pld, ec 3.4.13.9) activity assay was developed based on the determination of proline produced from enzymatic reaction through capillary electrophoresis (ce) with tris(2,2'-bipyridyl)ruthenium(11) [ru(bpy)(3)(2+)] electrochemiluminescence detection (ecl). a detection limit of 12.2 fmol (s/n = 3) for proline, corresponding to 1.22 x 10(-8) units of prolidase catalyzing for 1 min was achieved. pld activity determined by ce-ecl method was in agreement with that obtained from the classical chinard's one. ce-ecl showed its powerful resolving ability and selectivity as no sample pretreatmentwas needed and no interference existed. the clinical utility of this method was successfully demonstrated by its application to assay pld activity in the serum of diabetic patients in order to evaluate collagen degradation in diabetes mellitus (dm). the results indicated that enhanced collagen degradation occurred in dm. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000237456400012 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/15871]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yuan JP,Li T,Yin XB,et al. Characterization of prolidase activity using capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection and application to evaluate collagen degradation in diabetes mellitus[J]. analytical chemistry,2006,78(9):2934-2938. |
| APA | Yuan JP.,Li T.,Yin XB.,Guo L.,Jiang XZ.,...&Wang EK.(2006).Characterization of prolidase activity using capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection and application to evaluate collagen degradation in diabetes mellitus.analytical chemistry,78(9),2934-2938. |
| MLA | Yuan JP,et al."Characterization of prolidase activity using capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection and application to evaluate collagen degradation in diabetes mellitus".analytical chemistry 78.9(2006):2934-2938. |
入库方式： OAI收割
来源：长春应用化学研究所
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