Specific determination of As(V) by an acid phosphatase-polyphenol oxidase biosensor
文献类型：期刊论文
| 作者 | Cosnier S ; Mousty C ; Cui XQ ; Yang XR ; Dong SJ |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2006 |
| 卷号 | 78期号:14页码:4985-4989 |
| 关键词 | LAYERED DOUBLE HYDROXIDES ALKALINE-PHOSPHATASE ARSENIC SPECIATION ELECTRODE WATER INHIBITION SAMPLES ENZYME SENSOR CLAY |
| ISSN号 | 0003-2700 |
| 通讯作者 | cosnier s |
| 中文摘要 | an original amperometric biosensor based on the simultaneous entrapment of acid phosphatase (acp) and polyphenol oxidase (ppo) into anionic clays (layered double hydroxides) was developed for the specific detection of as(v). the functioning principle of the bienzyme electrode consisted of the successive hydrolysis of phenyl phosphate into phenol by acp, followed by the oxidation of phenol into o-quinone by ppo. the phenyl phosphate concentration was, thus, monitored by potentiostating the biosensor at -0.2 v vs ag/agcl to detect amperometrically the generated quinone. the detection of as(v) was based on its inhibitory effect on acp activity toward the hydrolysis of phenyl phosphate into phenol. the as(v) can be specifically determined in ph 6.0 acetate buffer without any interferences of as(iii) or phosphate, the detection limit being 2 nm or 0.15 ppb after an incubation step for 20 min. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239017700031 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/15875]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Cosnier S,Mousty C,Cui XQ,et al. Specific determination of As(V) by an acid phosphatase-polyphenol oxidase biosensor[J]. analytical chemistry,2006,78(14):4985-4989. |
| APA | Cosnier S,Mousty C,Cui XQ,Yang XR,&Dong SJ.(2006).Specific determination of As(V) by an acid phosphatase-polyphenol oxidase biosensor.analytical chemistry,78(14),4985-4989. |
| MLA | Cosnier S,et al."Specific determination of As(V) by an acid phosphatase-polyphenol oxidase biosensor".analytical chemistry 78.14(2006):4985-4989. |
入库方式： OAI收割
来源：长春应用化学研究所
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