Structural features of the L-argininamide-binding DNA aptamer studied with ESI-FTMS
文献类型：期刊论文
| 作者 | Guo XH ; Liu ZQ ; Liu SY ; Bentzley CM ; Bruist MF |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2006 |
| 卷号 | 78期号:20页码:7259-7266 |
| 关键词 | IONIZATION MASS-SPECTROMETRY DOUBLE-STRANDED OLIGODEOXYNUCLEOTIDES HIV-1 PACKAGING SIGNAL NONCOVALENT COMPLEXES ESCHERICHIA-COLI RNA STRUCTURES GAS-PHASE ION-TRAP ACID DUPLEX |
| ISSN号 | 0003-2700 |
| 通讯作者 | bruist mf |
| 中文摘要 | the 24-mer dna aptamer of harada and frankel ( harada, k.; frankel, a. d. embo j. 1995, 14, 5798-5811) that binds l-argininamide (l-arm) was studied by electrospray ionization fourier transform mass spectrometry (esi-ftms). this dna folds into a stem and loop such that the loop is able to engulf l-arm. as controls, two derivatives of the same base composition, one with the same stem but a scrambled loop and the other with no ability to form a secondary structure, were studied. the two dnas that could fold into stem-loop structures showed a more negatively charged distribution of ions than the linear control. this tendency was preserved in the presence of ligand; complexes expected to have more secondary structure had ions with more negative charges. distinct species corresponding to no, one, and two bound l-arm molecules were observed for each dna. the fractional peak intensities were fit to a straightforward binding model and binding constants were obtained. thus, esi-ftms can provide both qualitative and quantitative data regarding the structure of dna and its interactions with noncovalent ligands. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241219800026 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/15877]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Guo XH,Liu ZQ,Liu SY,et al. Structural features of the L-argininamide-binding DNA aptamer studied with ESI-FTMS[J]. analytical chemistry,2006,78(20):7259-7266. |
| APA | Guo XH,Liu ZQ,Liu SY,Bentzley CM,&Bruist MF.(2006).Structural features of the L-argininamide-binding DNA aptamer studied with ESI-FTMS.analytical chemistry,78(20),7259-7266. |
| MLA | Guo XH,et al."Structural features of the L-argininamide-binding DNA aptamer studied with ESI-FTMS".analytical chemistry 78.20(2006):7259-7266. |
入库方式： OAI收割
来源：长春应用化学研究所
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