Reversible B/Z-DNA transition under the low salt condition and non-B-form polydApolydT selectivity by a cubane-like europium-L-aspartic acid complex
文献类型：期刊论文
| 作者 | Zhang HY ; Yu HJ ; Ren JS ; Qu XG |
| 刊名 | biophysical journal
 |
| 出版日期 | 2006 |
| 卷号 | 90期号:9页码:3203-3207 |
| 关键词 | HANDED Z-DNA BINDING DYNAMICS |
| ISSN号 | 0006-3495 |
| 通讯作者 | qu xg |
| 中文摘要 | we report here that a cubane-like europium-l-aspartic acid complex at physiological ph can discriminate between dna structures as judged by the comparison of thermal denaturation, binding stoichiometry, temperature-dependent fluorescence enhancement, and circular dichroism and gel electrophoresis studies. this complex can selectively stabilize non-b-form dna polydapolydt but destabilize polydgdcpolydgdc and polydadtpolydadt. further studies show that this complex can convert b-form polydgdcpolydgdc to z-form under the low salt condition at physiological temperature 37 degrees c, and the transition is reversible, similar to rna polymerase, which turns unwound dna into z-dna and converts it back to b-dna after transcription. the potential uses of a left-handed helix-selective probe in biology are obvious. z-dna is a transient structure and does not exist as a stable feature of the double helix. therefore, probing this transient structure with a metal-amino acid complex under the low salt condition at physiological temperature would provide insights into their transitions in vivo and are of great interest. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236601100019 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/15905]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang HY,Yu HJ,Ren JS,et al. Reversible B/Z-DNA transition under the low salt condition and non-B-form polydApolydT selectivity by a cubane-like europium-L-aspartic acid complex[J]. biophysical journal,2006,90(9):3203-3207. |
| APA | Zhang HY,Yu HJ,Ren JS,&Qu XG.(2006).Reversible B/Z-DNA transition under the low salt condition and non-B-form polydApolydT selectivity by a cubane-like europium-L-aspartic acid complex.biophysical journal,90(9),3203-3207. |
| MLA | Zhang HY,et al."Reversible B/Z-DNA transition under the low salt condition and non-B-form polydApolydT selectivity by a cubane-like europium-L-aspartic acid complex".biophysical journal 90.9(2006):3203-3207. |
入库方式： OAI收割
来源：长春应用化学研究所
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