CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide
文献类型：期刊论文
| 作者 | Fang LY ; Kang JZ ; Yin XB ; Yang XR ; Wang EK |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006 |
| 卷号 | 27期号:22页码:4516-4522 |
| 关键词 | LASER-INDUCED FLUORESCENCE ELECTROGENERATED CHEMILUMINESCENCE DETECTION ELECTROPHORESIS-MASS SPECTROMETRY HUMAN ALPHA(1)-ACID GLYCOPROTEIN HIGHLY SULFATED CYCLODEXTRINS MONO-N-DEALKYLDISOPYRAMIDE PARTIAL-FILLING TECHNIQUE CAPILLARY-ELECTROPHORESIS AMINO-ACIDS LIQUID-CHROMATOGRAPHY |
| ISSN号 | 0173-0835 |
| 通讯作者 | wang ek |
| 中文摘要 | ce with electrochemiluminescence, (ecl) detection technique was successfully applied for the chiral separation of a kind of class ia antiarrhythmic racemic drug. to the best of our knowledge, this is the first report of ecl detection used in chiral ce. to get better detection sensitivity and good enantioresolution at the same time, the conditions of capillary inlet and outlet buffer were systematically optimized. unlike the traditional chiral separation method, the buffers we used in the capillary inlet and outlet differed from each other in terms of buffer ph, ionic strength, type of bge as well as buffer composition. under the optimum conditions, baseline enantioseparation and highly sensitive detection of the enantiomers were achieved. wide linear relationship of each enantiomer was achieved in the range of 5 x 10(-7) to 2 x 10(-5) mol/l with relative coefficients of 0.996 and 0.997, respectively. the detection limits were estimated to be 8 x 10(-8) and 1.0 x 10(-7) mol/l (s/n = 3) for the enantiomers, respectively. in addition, a successful application of this new method to the chiral separation of the racemic drug in spiked plasma samples confirmed the validity and applicability of the chiral ce-ecl method. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242695900015 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16031]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Fang LY,Kang JZ,Yin XB,et al. CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide[J]. electrophoresis,2006,27(22):4516-4522. |
| APA | Fang LY,Kang JZ,Yin XB,Yang XR,&Wang EK.(2006).CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide.electrophoresis,27(22),4516-4522. |
| MLA | Fang LY,et al."CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide".electrophoresis 27.22(2006):4516-4522. |
入库方式： OAI收割
来源：长春应用化学研究所
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