Effect of DNA flanking sequence on charge transport in short DNA duplexes
文献类型：期刊论文
| 作者 | Li X ; Peng YH ; Ren JS ; Qu XG |
| 刊名 | biochemistry
 |
| 出版日期 | 2006 |
| 卷号 | 45期号:45页码:13543-13550 |
| 关键词 | MEDIATED ELECTRON-TRANSFER GOLD ELECTRODES BASE-STACKING B-FORM ELECTROCHEMICAL REACTION DISTANCE DEPENDENCE HOPPING MECHANISM ANTICANCER DRUG HOLE TRANSPORT GLASSY-CARBON |
| ISSN号 | 0006-2960 |
| 通讯作者 | qu xg |
| 中文摘要 | several factors can influence charge transport (ct)-mediated dna, such as sequence, distance, base stacking, base pair mismatch, conformation, tether length, etc. however, the dna context effect or how flanking sequences influence redox active drugs in the dna ct reaction and later in dna enzymatic repair and synthesis is still not well understood. the set of seven dna molecules in this study have been characterized well for the study of flanking sequence effects. these dna duplexes are formed from self-complementary strands and contain the common central four-base sequence 5'-a-g-c-t-3', flanked on both sides by either (at)(n) or (aa)(n) (n = 2, 3, or 4) or aa(at)(2). uv-vis, fluorescence, uv melting, circular dichroism, and cyclic voltammetry experiments were used to study the flanking sequence effect on ct-mediated dna by using daunomycin or adriamycin cross-linked with these seven dna molecules. our results showed that charge transport was related to the flanking sequence, dna melting free energy, and ionic strength. for (aa)(n) or (at)(n) species of the same length, (aa)(n) series were more stable and more efficient ct was observed through the (aa)(n) series. the same trend was observed for (aa)(n) and (at)(n) series at different ionic strengths, further supporting the idea that flanking sequence can result in different base stacking and modulate charge transport through these seven dna molecules. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241808300015 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16033]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li X,Peng YH,Ren JS,et al. Effect of DNA flanking sequence on charge transport in short DNA duplexes[J]. biochemistry,2006,45(45):13543-13550. |
| APA | Li X,Peng YH,Ren JS,&Qu XG.(2006).Effect of DNA flanking sequence on charge transport in short DNA duplexes.biochemistry,45(45),13543-13550. |
| MLA | Li X,et al."Effect of DNA flanking sequence on charge transport in short DNA duplexes".biochemistry 45.45(2006):13543-13550. |
入库方式： OAI收割
来源：长春应用化学研究所
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