Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite
文献类型：期刊论文
| 作者 | Liu Y ; Qu XH ; Guo HW ; Chen HJ ; Liu BF ; Dong SJ |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2006 |
| 卷号 | 21期号:12页码:2195-2201 |
| 关键词 | ELECTRON-TRANSFER GOLD ELECTRODE REDUCTION FILMS SOLUBILIZATION INTEGRATION PROTEINS DIOXYGEN PHENOLS CATHODE |
| ISSN号 | 0956-5663 |
| 通讯作者 | dong sj |
| 中文摘要 | the carbon nanotubes-chitosan (cnts-cs) composite provides a suitable biosensing matrix due to its good conductivity, high stability, and good biocompatibility. enzymes can be firmly incorporated into the matrix without the aid of other cross-linking reagents. the composite is easy to form insoluble film in solution above ph 6.3. based on this, a facilely fabricated amperometric biosensor by entrapping laccase into the cnts-cs composite film has been developed. at ph 6.0, the fungi laccase incorporated into the composite film remains better catalytic activity than that dissolved in solution. the system is in favor of the accessibility of substrate to the active site of laccase, thus the affinity to substrates is improved greatly, such as 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (abts), catechol, and 0, with k. values of 19.86 mu m, 9.43 mu m, and 3.22 mm, respectively. the major advantages of the as-prepared biosensor are: detecting different substrates (abts, catechol, and 02), possessing high affinity and sensitivity, durable long-term stability, and facile preparation procedure. on the other hand, the system can be applied in fabrication of biofuel cells as the cathodic catalysts based on its good electrocatalysis for oxygen reduction. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000237613700001 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16077]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu Y,Qu XH,Guo HW,et al. Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite[J]. biosensors & bioelectronics,2006,21(12):2195-2201. |
| APA | Liu Y,Qu XH,Guo HW,Chen HJ,Liu BF,&Dong SJ.(2006).Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite.biosensors & bioelectronics,21(12),2195-2201. |
| MLA | Liu Y,et al."Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite".biosensors & bioelectronics 21.12(2006):2195-2201. |
入库方式： OAI收割
来源：长春应用化学研究所
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