Capillary electrophoresis with electrochemiluminescence detection for measurement of aspartate aminotransferase and alanine aminotransferase activities in biofluids
文献类型：期刊论文
| 作者 | Li T ; Yuan J ; Yin JY ; Zhang ZW ; Wang EK |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2006 |
| 卷号 | 1134期号:1-2页码:311-316 |
| 关键词 | ALCOHOLIC LIVER-DISEASE CONTACTLESS CONDUCTIVITY DETECTION PERFORMANCE LIQUID-CHROMATOGRAPHY CATALYTIC ACTIVITY CONCENTRATIONS UNDERIVATIZED AMINO-ACIDS ZONE-ELECTROPHORESIS CHEMILUMINESCENCE DETECTION CELL-LINE SEPARATION RATIO |
| ISSN号 | 0021-9673 |
| 通讯作者 | wang ek |
| 中文摘要 | a new sensitive assay for aspartate aminotransterase (ast) and alanine aminotransferase (alt) activities in biofluids was developed, based on the separation and detection of alanine, glutamate, and aspartate using capillary electrophoresis (ce) with electrochemiluminescence (ecl) detection. the three amino acids were separated in 5 mm phosphate of ph 2.1 as background electrolyte, and detected on a 500 mu m platinum disk electrode at 1.2 v (versus ag/agcl) in the presence of 10 mm tris(2,2'-bipyridyl)ruthenium(ii) dissolved in 80 mm phosphate of ph 10.5. a mass detection limit of 37.3 fmol (or 81.5 fmol) for glutamate, corresponding to the product in the enzyme reaction catalyzed by 1.24 x 10(-9) u ast (or 2.72 x 10(-9) u alt) in a 30 min reaction period, was achieved. this assay was applied to investigate the cytotoxicity effect of ethanol on hepg2 cells and differentiating nonalcoholic steatohepatitis (nash) from alcoholic liver disease, indicating that the technique is promising for the application in the cell biological and clinical fields. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242059400038 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16135]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Yuan J,Yin JY,et al. Capillary electrophoresis with electrochemiluminescence detection for measurement of aspartate aminotransferase and alanine aminotransferase activities in biofluids[J]. journal of chromatography a,2006,1134(1-2):311-316. |
| APA | Li T,Yuan J,Yin JY,Zhang ZW,&Wang EK.(2006).Capillary electrophoresis with electrochemiluminescence detection for measurement of aspartate aminotransferase and alanine aminotransferase activities in biofluids.journal of chromatography a,1134(1-2),311-316. |
| MLA | Li T,et al."Capillary electrophoresis with electrochemiluminescence detection for measurement of aspartate aminotransferase and alanine aminotransferase activities in biofluids".journal of chromatography a 1134.1-2(2006):311-316. |
入库方式： OAI收割
来源：长春应用化学研究所
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