Electrogenerated chemiluminescence biosensor based on Ru(bpy)(3)(2+) and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material
文献类型：期刊论文
| 作者 | Zhang LH ; Xu ZA ; Dong SJ |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2006 |
| 卷号 | 575期号:1页码:52-56 |
| 关键词 | HYDROGEN-PEROXIDE BIOSENSOR SOL-GEL CHEMI-LUMINESCENCE ALCOHOL-DEHYDROGENASE GLUCOSE BIOSENSOR HYBRID MATERIAL FILMS ELECTROCHEMILUMINESCENCE ELECTRODES NAFION |
| ISSN号 | 0003-2670 |
| 通讯作者 | dong sj |
| 中文摘要 | a new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ecl reagent ru(bpy)(3)(2+) and alcohol dehydrogenase in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) (pss) organically modified composite material. the component pss was used to immobilize ecl reagent ru(bpy)(3)(2+) by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol-gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. such biosensor combined enzymatic selectivity with the sensitivity of ecl detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. the detection limit was 9.3 x 10(-6) m for alcohol (s/n = 3) with a linear range from 2.79 x 10(-5) to 5.78 x 10(-2) m. with ecl detection, the biosensor exhibited wide linear range, high sensitivity and good stability. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239674600008 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16247]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang LH,Xu ZA,Dong SJ. Electrogenerated chemiluminescence biosensor based on Ru(bpy)(3)(2+) and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material[J]. analytica chimica acta,2006,575(1):52-56. |
| APA | Zhang LH,Xu ZA,&Dong SJ.(2006).Electrogenerated chemiluminescence biosensor based on Ru(bpy)(3)(2+) and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material.analytica chimica acta,575(1),52-56. |
| MLA | Zhang LH,et al."Electrogenerated chemiluminescence biosensor based on Ru(bpy)(3)(2+) and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material".analytica chimica acta 575.1(2006):52-56. |
入库方式： OAI收割
来源：长春应用化学研究所
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