Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity
文献类型：期刊论文
| 作者 | Wang J ; Hong A ; Ren JS ; Sun FY ; Shi YJ ; Liu K ; Xie QL ; Dai Y ; Li ZY ; Chen Y |
| 刊名 | journal of biotechnology
 |
| 出版日期 | 2006 |
| 卷号 | 121期号:4页码:442-447 |
| 关键词 | FIBROBLAST-GROWTH-FACTOR SELF-ASSOCIATION HEPARIN FACTOR-2 BINDING |
| ISSN号 | 0168-1656 |
| 通讯作者 | wang j |
| 中文摘要 | fibroblast growth factor-2 (fgf-2) is a multifunctional polypeptide that affects many cellular functions and phenomena. the wild-type recombinant human fibroblast growth factor rhfgf-2(w) and the mutant c78sc96s rhfgf-2(m) were expressed in escherichia coli and their products were purified. the results by the means of fluorescence spectroscopy and cd spectrums, suggested that due to its decreased hydrophobicity rhfgf-2 is not deposited as an inclusion body. the mitogenic activity of the expressed rhfgf-2(m) on 3t3 fibroblasts was shown to be 10-fold more than the expressed rhfgf-2(w) of which the biological activity was a little less than that of the standard rhbfgf(w), indicating that the increased biological activity was due to the change of its secondary structure, dimerization and affinity binding to fgf receptor (fgfr). |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235697100002 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16261]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang J,Hong A,Ren JS,et al. Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity[J]. journal of biotechnology,2006,121(4):442-447. |
| APA | Wang J.,Hong A.,Ren JS.,Sun FY.,Shi YJ.,...&Chen Y.(2006).Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity.journal of biotechnology,121(4),442-447. |
| MLA | Wang J,et al."Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity".journal of biotechnology 121.4(2006):442-447. |
入库方式： OAI收割
来源：长春应用化学研究所
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