Application of electrochemical impedance spectroscopy for monitoring allergen-antibody reactions using gold nanoparticle-based biomolecular immobilization method
文献类型：期刊论文
| 作者 | Huang HZ ; Liu ZG ; Yang XR |
| 刊名 | analytical biochemistry
 |
| 出版日期 | 2006 |
| 卷号 | 356期号:2页码:208-214 |
| 关键词 | FC-RECEPTORS IGE IMMUNOSENSOR AFFINITY PROTEIN IMMUNOASSAY ELECTRODE BIOSENSORS MONOLAYER BINDING |
| ISSN号 | 0003-2697 |
| 通讯作者 | liu zg |
| 中文摘要 | gold nanoparticles were used to enhance the immobilization amount and retain the immunoactivity of recombinant dust mite allergen der f2 immobilized on a glassy carbon electrode (gce). the interaction between allergen and antibody was studied by electrochemical impedance spectroscopy (eis). self-assembled au colloid layer (phi = 16 nm) deposited on (3-mercaptopropyl)trimethoxysilane (mpts)-modified gce offered a basis to control the immobilization of allergen der f2. the impedance measurements were based on the charge transfer kinetics of the [fe(cn)(6)](3-/4-) redox pair, compared with bare gce, the immobilization of allergen der f2 and the allergen-antibody interaction that occurred on the electrode surface altered the interfacial electron transfer resistance and thereby slowed down the charge transfer kinetics by reducing the active area of the electrode or by preventing the redox species in electrolyte solution from approaching the electrode. the interactions of allergen with various concentrations of monoclonal antibody were also monitored through the change of impedance response. the results showed that the electron transfer resistance increased with increasing concentrations of monoclonal antibody. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240523700006 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16263]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Huang HZ,Liu ZG,Yang XR. Application of electrochemical impedance spectroscopy for monitoring allergen-antibody reactions using gold nanoparticle-based biomolecular immobilization method[J]. analytical biochemistry,2006,356(2):208-214. |
| APA | Huang HZ,Liu ZG,&Yang XR.(2006).Application of electrochemical impedance spectroscopy for monitoring allergen-antibody reactions using gold nanoparticle-based biomolecular immobilization method.analytical biochemistry,356(2),208-214. |
| MLA | Huang HZ,et al."Application of electrochemical impedance spectroscopy for monitoring allergen-antibody reactions using gold nanoparticle-based biomolecular immobilization method".analytical biochemistry 356.2(2006):208-214. |
入库方式： OAI收割
来源：长春应用化学研究所
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