A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized in DNA films on a gold electrode
文献类型：期刊论文
| 作者 | Song YH ; Wang L ; Ren CB ; Zhu GY ; Li Z |
| 刊名 | sensors and actuators b-chemical
 |
| 出版日期 | 2006 |
| 卷号 | 114期号:2页码:1001-1006 |
| 关键词 | DIRECT ELECTROCHEMISTRY GRAPHITE ELECTRODE BIOSENSOR NANOPARTICLES MONOLAYERS PROTEINS AU(111) NETWORK |
| ISSN号 | 0925-4005 |
| 通讯作者 | li z |
| 中文摘要 | a novel third-generation hydrogen peroxide (h2o2) biosensor was developed by immobilizing horseradish peroxidase (hrp) on a biocompatible gold electrode modified with a well-ordered, self-assembled dna film. cysteamine was first self-assembled on a gold electrode to provide an interface for the assembly of dna molecules. then dna was chemisorbed onto the self-assembled monolayers (sams) of cysteamine to form a network by controlling dna concentration. the dna-network film obtained provided a biocompatible microenvironment for enzyme molecules, greatly amplified the coverage of hrp molecules on the electrode surface, and most importantly could act as a charge carrier which facilitated the electron transfer between hrp and the electrode. finally, hrp was adsorbed on the dna-network film. the process of the biosensor construction was followed by atomic force microscopy (afm). voltammetric and time-based amperometric techniques were employed to characterize the properties of the biosensor derived. the enzyme electrode achieved 95% of the steady-state current within 2 s and had a 0.5 mu mol l(-1) detection limit of h2o2. furthermore, the biosensor showed high sensitivity, good reproducibility, and excellent long-term stability. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236651600061 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16269]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Song YH,Wang L,Ren CB,et al. A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized in DNA films on a gold electrode[J]. sensors and actuators b-chemical,2006,114(2):1001-1006. |
| APA | Song YH,Wang L,Ren CB,Zhu GY,&Li Z.(2006).A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized in DNA films on a gold electrode.sensors and actuators b-chemical,114(2),1001-1006. |
| MLA | Song YH,et al."A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized in DNA films on a gold electrode".sensors and actuators b-chemical 114.2(2006):1001-1006. |
入库方式： OAI收割
来源：长春应用化学研究所
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