The interaction of recombinant Pseudomonas aeruginosa exotoxin A with DNA and mimic biomembrane investigated by surface plasmon resonance and electrochemical methods
文献类型：期刊论文
| 作者 | Li AX ; Sun ZZ ; Ma Y ; Yang F ; Ouyang HS ; Yang XR |
| 刊名 | talanta
 |
| 出版日期 | 2006 |
| 卷号 | 70期号:2页码:330-335 |
| 关键词 | GENE DELIVERY PHOSPHOLIPID MONOLAYERS BILAYER-MEMBRANES CARBON NANOTUBES LIPID-BILAYERS TOXIN PROTEIN BIOSENSOR POLYMERS DENSITY |
| ISSN号 | 0039-9140 |
| 通讯作者 | yang xr |
| 中文摘要 | based on the multidomain structure of pseudomonas aeruginosa exotoxin a, a fusion protein termed rpea has been constructed, which is expected to serve as a gene carrier in vitro. the expression and purification of rpea are described. the basal properties of rpea as a gene carrier are evaluated by investigating its interaction with plasmid dna and mimic biomembrane by surface plasmon resonance (spr) and electrochemical methods. rpea is proved to be able to bind with plasmid dna with high affinity. it can also interact with lipid membrane and increase permeability of the membrane, so the probe molecules can easily reach the gold surface and exhibit the electrochemical response. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240418700015 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16331]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li AX,Sun ZZ,Ma Y,et al. The interaction of recombinant Pseudomonas aeruginosa exotoxin A with DNA and mimic biomembrane investigated by surface plasmon resonance and electrochemical methods[J]. talanta,2006,70(2):330-335. |
| APA | Li AX,Sun ZZ,Ma Y,Yang F,Ouyang HS,&Yang XR.(2006).The interaction of recombinant Pseudomonas aeruginosa exotoxin A with DNA and mimic biomembrane investigated by surface plasmon resonance and electrochemical methods.talanta,70(2),330-335. |
| MLA | Li AX,et al."The interaction of recombinant Pseudomonas aeruginosa exotoxin A with DNA and mimic biomembrane investigated by surface plasmon resonance and electrochemical methods".talanta 70.2(2006):330-335. |
入库方式： OAI收割
来源：长春应用化学研究所
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