In vitro study on the binding of neutral red to bovine serum albumin by molecular spectroscopy
文献类型：期刊论文
| 作者 | Shang L ; Jiang XU ; Dong SJ |
| 刊名 | journal of photochemistry and photobiology a-chemistry
 |
| 出版日期 | 2006 |
| 卷号 | 184期号:1-2页码:93-97 |
| 关键词 | PHYSICOCHEMICAL PROPERTIES DRUG-BINDING FLUORESCENCE PORPHYRINS ABSORPTION MECHANISMS PROTEINS ACID DNA |
| ISSN号 | 1010-6030 |
| 通讯作者 | dong sj |
| 中文摘要 | in this paper, the binding of neutral red (nr) to bovine serum albumin (bsa) under physiological conditions has been studied by spectroscopy method including fluorescence, circular dichroism (cd) and fourier transform infrared (ft-ir) spectroscopy. the stern-volmer fluorescence quenching constant (k-sv), binding constant (k-b) and the number of binding sites (it) were measured by fluorescence quenching method. fluorescence experiments were also performed at different ionic strengths. it was found k-sv was ionic strength dependent, which indicated the electrostatic interactions were part of the binding forces. the distance r between donor (bsa) and acceptor (nr) was obtained according to foster's non-radiative energy transfer theory. cd spectroscopy and ft-ir spectroscopy were used to investigate the structural information of bsa molecules on the binding of nr, and the results showed no change of bsa conformation in our experimental conditions. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243145200012 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16351]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Shang L,Jiang XU,Dong SJ. In vitro study on the binding of neutral red to bovine serum albumin by molecular spectroscopy[J]. journal of photochemistry and photobiology a-chemistry,2006,184(1-2):93-97. |
| APA | Shang L,Jiang XU,&Dong SJ.(2006).In vitro study on the binding of neutral red to bovine serum albumin by molecular spectroscopy.journal of photochemistry and photobiology a-chemistry,184(1-2),93-97. |
| MLA | Shang L,et al."In vitro study on the binding of neutral red to bovine serum albumin by molecular spectroscopy".journal of photochemistry and photobiology a-chemistry 184.1-2(2006):93-97. |
入库方式： OAI收割
来源：长春应用化学研究所
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