Single-molecule dynamics reveals cooperative binding-folding in protein recognition
文献类型：期刊论文
| 作者 | Wang J ; Lu Q ; Lu HP |
| 刊名 | plos computational biology
 |
| 出版日期 | 2006 |
| 卷号 | 2期号:7页码:842-852 |
| 关键词 | TRANSITION-STATE ENSEMBLE ALDRICH-SYNDROME PROTEIN MONTE-CARLO SIMULATIONS ENERGY LANDSCAPE CONFORMATIONAL DYNAMICS CONTACT ORDER MECHANISM CDC42 TOPOLOGY FUNNELS |
| ISSN号 | 1553-734x |
| 通讯作者 | wang j |
| 中文摘要 | the study of associations between two biomolecules is the key to understanding molecular function and recognition. molecular function is often thought to be determined by underlying structures. here, combining a single-molecule study of protein binding with an energy-landscape-inspired microscopic model, we found strong evidence that biomolecular recognition is determined by flexibilities in addition to structures. our model is based on coarse-grained molecular dynamics on the residue level with the energy function biased toward the native binding structure ( the go model). with our model, the underlying free-energy landscape of the binding can be explored. there are two distinct conformational states at the free-energy minimum, one with partial folding of cbd itself and significant interface binding of cbd to cdc42, and the other with native folding of cbd itself and native interface binding of cbd to cdc42. this shows that the binding process proceeds with a significant interface binding of cbd with cdc42 first, without a complete folding of cbd itself, and that binding and folding are then coupled to reach the native binding state. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239494100017 |
| 公开日期 | 2010-08-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/16979]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang J,Lu Q,Lu HP. Single-molecule dynamics reveals cooperative binding-folding in protein recognition[J]. plos computational biology,2006,2(7):842-852. |
| APA | Wang J,Lu Q,&Lu HP.(2006).Single-molecule dynamics reveals cooperative binding-folding in protein recognition.plos computational biology,2(7),842-852. |
| MLA | Wang J,et al."Single-molecule dynamics reveals cooperative binding-folding in protein recognition".plos computational biology 2.7(2006):842-852. |
入库方式： OAI收割
来源：长春应用化学研究所
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