Direct tris(2,2 '-bipyridyl)ruthenium(II) electrochemiluminescence detection of polyamines separated by capillary electrophoresis
文献类型：期刊论文
| 作者 | Liu JF ; Yang XR ; Wang EK |
| 刊名 | electrophoresis
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:18页码:3131-3138 |
| 关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY RU(BPY)(3)(3+)-BASED CHEMILUMINESCENCE DETECTION MICELLAR ELECTROKINETIC CHROMATOGRAPHY INDIRECT PHOTOMETRIC DETECTION ELECTROGENERATED CHEMILUMINESCENCE ZONE-ELECTROPHORESIS BIOGENIC-AMINES CANCER COLUMN MONOACETYLPOLYAMINES |
| ISSN号 | 0173-0835 |
| 通讯作者 | wang ek |
| 中文摘要 | capillary electrophoresis (ce) with tris(2,2'-bipyridyl) ruthenium (ii) (ru(bpy)(3)(2+)) electrochemiluminescence (ecl) detection technique was developed for the analysis of four polyamines (putrescine (put), cadaverine (cad), spermidine (spd), and spermine (spm)) analysis. the four polyamines contain different amine groups, which have different ecl activity. there are several parameters which influence the resolution and ecl peak intensities, including the buffer ph and concentrations, separation voltage, sample injection, electrode materials, and ru(bpy)(3)(2+) concentrations. polyamines are separated by capillary zone electrophoresis in an uncoated fused-silica capillary (50 cm x 25 mum (id) filled with acidic phosphate buffer (200 mmol/l phosphate, ph 2.0) - 1 mol/l phosphoric acid (9:1 v/v) and a separation voltage of 5 kv (25 mua), with end-column ru(bpy)(3)(2+) ecl detection. a 5 mmol/l ru(bpy)(3)(2+) solution plus 200 mmol/l phosphate buffer (ph 11.0) is added into the reagent reservoir. the calibration curve is linear over a concentration range of two or three orders of magnitude for the polyamines. the analysis time is less than 25 min. detection limits for put and cad are 1.9 x 10(-7) mol/l and 7.6 x 10(-9) mol/l for spd and spm, respectively. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185897100005 |
| 公开日期 | 2010-09-13 |
| 源URL | [http://202.98.16.49/handle/322003/17511]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu JF,Yang XR,Wang EK. Direct tris(2,2 '-bipyridyl)ruthenium(II) electrochemiluminescence detection of polyamines separated by capillary electrophoresis[J]. electrophoresis,2003,24(18):3131-3138. |
| APA | Liu JF,Yang XR,&Wang EK.(2003).Direct tris(2,2 '-bipyridyl)ruthenium(II) electrochemiluminescence detection of polyamines separated by capillary electrophoresis.electrophoresis,24(18),3131-3138. |
| MLA | Liu JF,et al."Direct tris(2,2 '-bipyridyl)ruthenium(II) electrochemiluminescence detection of polyamines separated by capillary electrophoresis".electrophoresis 24.18(2003):3131-3138. |
入库方式： OAI收割
来源：长春应用化学研究所
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