Context and conformation dictate function of a transcription antitermination switch
文献类型：期刊论文
| 作者 | Xia TB ; Frankel A ; Takahashi TT ; Ren JS ; Roberts RW |
| 刊名 | nature structural biology
 |
| 出版日期 | 2003 |
| 卷号 | 10期号:10页码:812-819 |
| 关键词 | RNA-BINDING PEPTIDES LAMBDA-N-PROTEIN PHAGE-LAMBDA BACTERIOPHAGE-LAMBDA PROCESSIVE ANTITERMINATION GENE-EXPRESSION IN-VITRO COMPLEX POLYMERASE ELONGATION |
| ISSN号 | 1072-8368 |
| 通讯作者 | roberts rw |
| 中文摘要 | in bacteriophage, transcription elongation is regulated by the n protein, which binds a nascent mrna hairpin ( termed boxb) and enables rna polymerase to read through distal terminators. we have examined the structure, energetics and in vivo function of a number of n boxb complexes derived from in vitro protein selection. trp18 fully stacks on the rna loop in the wild-type structure, and can become partially or completely unstacked when the sequence context is changed three or four residues away, resulting in a recognition interface in which the best binding residues depend on the sequence context. notably, in vivo antitermination activity correlates with the presence of a stacked aromatic residue at position 18, but not with n boxb binding affinity. our work demonstrates that rna polymerase responds to subtle conformational changes in cis-acting regulatory complexes and that approximation of components is not sufficient to generate a fully functional transcription switch. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185624900012 |
| 公开日期 | 2010-09-13 |
| 源URL | [http://202.98.16.49/handle/322003/17807]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xia TB,Frankel A,Takahashi TT,et al. Context and conformation dictate function of a transcription antitermination switch[J]. nature structural biology,2003,10(10):812-819. |
| APA | Xia TB,Frankel A,Takahashi TT,Ren JS,&Roberts RW.(2003).Context and conformation dictate function of a transcription antitermination switch.nature structural biology,10(10),812-819. |
| MLA | Xia TB,et al."Context and conformation dictate function of a transcription antitermination switch".nature structural biology 10.10(2003):812-819. |
入库方式： OAI收割
来源：长春应用化学研究所
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