Real-time immunoassay of ferritin using surface plasmon resonance biosensor
文献类型：期刊论文
| 作者 | Cui XQ ; Yang F ; Sha YF ; Yang XR |
| 刊名 | talanta
 |
| 出版日期 | 2003 |
| 卷号 | 60期号:1页码:53-61 |
| 关键词 | BIOSPECIFIC INTERACTION ANALYSIS SERUM FERRITIN IRON IMMOBILIZATION PROTEINS SENSORS SITE |
| ISSN号 | 0039-9140 |
| 通讯作者 | yang xr |
| 中文摘要 | a surface plasmon resonance (spr) biosensor was used for the first time to determine the concentration of ferritin in both hbs-ep buffer and serum. the monoclonal antibody was immobilized on the carboxymethyl dextran-modified gold surface by an amine coupling method. the interaction of antibody with antigen was monitored in real-time. the signal was enhanced by sandwich amplification strategy to improve the sensitivity and specificity of the immunoassay, especially in serum. the linear range of the assay in serum is over 30-200 ng ml with the detection limit of 28 ng ml(-1). the sensitivity, specificity, and reproducibility of the assay are satisfactory. the analyte and enhancement antibody-binding surface could be regenerated by ph 2.0 glycine-hcl buffer and the same antibody-immobilized surface could be used for more than 50 cycles of ferritin binding and regeneration. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183183800008 |
| 公开日期 | 2010-09-13 |
| 源URL | [http://202.98.16.49/handle/322003/17893]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Cui XQ,Yang F,Sha YF,et al. Real-time immunoassay of ferritin using surface plasmon resonance biosensor[J]. talanta,2003,60(1):53-61. |
| APA | Cui XQ,Yang F,Sha YF,&Yang XR.(2003).Real-time immunoassay of ferritin using surface plasmon resonance biosensor.talanta,60(1),53-61. |
| MLA | Cui XQ,et al."Real-time immunoassay of ferritin using surface plasmon resonance biosensor".talanta 60.1(2003):53-61. |
入库方式： OAI收割
来源：长春应用化学研究所
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