Conformation change of horseradish peroxidase in lipid membrane
文献类型：期刊论文
| 作者 | Tang JL ; Jiang JG ; Song YH ; Peng ZQ ; Wu ZY ; Dong SJ ; Wang EK |
| 刊名 | chemistry and physics of lipids
 |
| 出版日期 | 2002 |
| 卷号 | 120期号:1-2页码:119-129 |
| 关键词 | REFLECTION FLUORESCENCE MICROSCOPY SUPPORTED PLANAR MEMBRANES ATOMIC-FORCE MICROSCOPE ELECTRON-TRANSFER SURFACTANT FILMS DIRECT ELECTROCHEMISTRY PHOSPHOLIPID-BILAYERS LANGMUIR-BLODGETT MYOGLOBIN ADSORPTION |
| ISSN号 | 0009-3084 |
| 通讯作者 | wang ek |
| 中文摘要 | the electrochemical behavior of horseradish peroxidase (hrp) in the dimyristoyl phosphatidylcholine (dmpc) bilayer on the glassy carbon (gc) electrode was studied by cyclic voltammetry. the direct electron transfer of hrp was observed in the dmpc bilayer. only a small cathodic peak was observed for hrp on the bare gc electrode. the electron transfer of hrp in the dmpc membrane is facilitated by dmpc membrane. uv-vis and circular dichroism (cd) spectroscopy were used to study the interaction between hrp and dmpc membrane. on binding to the dmpc membrane the secondary structure of hrp remains unchanged while there is a substantial change in the conformation of the heme active site. tapping mode atomic force microscopy (afm) was first applied for the investigation on the structure of hrp adsorbed on supported phospholipid bilayer on the mica and on the bare mica. hrp molecules adsorb and aggregate on the mica without dmpc bilayer. the aggregation indicates an attractive interaction among the adsorbed molecules. the molecules are randomly distributed in the dmpc bilayer. the adsorption of hrp in the dmpc bilayer changes drastically the domains and defects in the dmpc bilayer due to a strong interaction between hrp and dmpc films. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000180580100011 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18125]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tang JL,Jiang JG,Song YH,et al. Conformation change of horseradish peroxidase in lipid membrane[J]. chemistry and physics of lipids,2002,120(1-2):119-129. |
| APA | Tang JL.,Jiang JG.,Song YH.,Peng ZQ.,Wu ZY.,...&Wang EK.(2002).Conformation change of horseradish peroxidase in lipid membrane.chemistry and physics of lipids,120(1-2),119-129. |
| MLA | Tang JL,et al."Conformation change of horseradish peroxidase in lipid membrane".chemistry and physics of lipids 120.1-2(2002):119-129. |
入库方式： OAI收割
来源：长春应用化学研究所
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