Preparation and properties of abzyme with thyroxine deiodinase
文献类型：期刊论文
| 作者 | Sun LW ; Liu LY ; Zhao DQ |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2002 |
| 卷号 | 23期号:12页码:2217-2220 |
| 关键词 | I IODOTHYRONINE DEIODINASE 5'-DEIODINASE ANTIBODY ENZYME |
| ISSN号 | 0251-0790 |
| 通讯作者 | zhao dq |
| 中文摘要 | the abzyme (se-6e8) with a higher thyroxine deiodinase activity was prepared by modifying the serine residues of monoclonal antibody (6e8)with phenylmethanesulfonyl fluoride and sodium hydrogen selenide, and the 6e8 against o-methyl-t-4, which is a kind of thyroxine derivatives and was taken as a hapten for the first time. two bands were found corresponding to the 5.5 kd heavy chain and the 2.7 kd light chain respectively by sds-page. the characteristics of dissociation constants, ph, and temperature were also studied. the results show that the activity of se-6e8 is 2 010 u/mumol protein, and the proper temperature and ph of the catalytic reactions is 57 degreesc and 8.2 respectively. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000179776400001 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18165]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun LW,Liu LY,Zhao DQ. Preparation and properties of abzyme with thyroxine deiodinase[J]. chemical journal of chinese universities-chinese,2002,23(12):2217-2220. |
| APA | Sun LW,Liu LY,&Zhao DQ.(2002).Preparation and properties of abzyme with thyroxine deiodinase.chemical journal of chinese universities-chinese,23(12),2217-2220. |
| MLA | Sun LW,et al."Preparation and properties of abzyme with thyroxine deiodinase".chemical journal of chinese universities-chinese 23.12(2002):2217-2220. |
入库方式： OAI收割
来源：长春应用化学研究所
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