Capillary liquid chromatographic-high-resolution mass spectrometric analysis of ribonucleotides
文献类型：期刊论文
| 作者 | Cai ZW ; Song FR ; Yang MS |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2002 |
| 卷号 | 976期号:1-2页码:135-143 |
| 关键词 | NUCLEOTIDES SENSITIVITY METABOLITES |
| ISSN号 | 0021-9673 |
| 通讯作者 | cai zw |
| 中文摘要 | a method of capillary hplc-high-resolution ms was developed for the trace analysis of atp, gtp, datp and dgtp dimethylhexylamine (dmha) was used as ion-pairing agent for the hplc retention and separation of the nucleotides and positive ion electrospray time-of-flight ms was used for the detection. the application of capillary hplc allowed minimal usage of dmha while providing excellent peak retention and resolution, which significantly reduced the ion suppression in electrospray ionization-ms analysis and thus increased the sensitivity. adduct ions of nucleotides and dmha were used as quantitative ions in order to achieve the best sensitivity. dmha concentration at 5 mm in the aqueous mobile phase at ph 7 was found to be the optimal conditions for the c is capillary column. the method was applied to determine atp level in cultured c6 glioma cells that were treated with toxic concentrations of zn. the results showed that the cellular atp level decreased from 2.7 pmol/cell (<10% cell death) in average control cell samples to 0.36 pmol/cell as the concentration of zn increased to 120 mg/l (>35% cell death) in culture medium. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000179110100016 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18217]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Cai ZW,Song FR,Yang MS. Capillary liquid chromatographic-high-resolution mass spectrometric analysis of ribonucleotides[J]. journal of chromatography a,2002,976(1-2):135-143. |
| APA | Cai ZW,Song FR,&Yang MS.(2002).Capillary liquid chromatographic-high-resolution mass spectrometric analysis of ribonucleotides.journal of chromatography a,976(1-2),135-143. |
| MLA | Cai ZW,et al."Capillary liquid chromatographic-high-resolution mass spectrometric analysis of ribonucleotides".journal of chromatography a 976.1-2(2002):135-143. |
入库方式： OAI收割
来源：长春应用化学研究所
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