Surface-enhanced resonance Raman spectroscopic study of yeast iso-1-cytochrome c and its mutant
文献类型:期刊论文
作者 | Zheng JW ; Zhou Q ; Zhou YG ; Lu TH ; Cotton TM ; Chumanov G |
刊名 | journal of electroanalytical chemistry |
出版日期 | 2002 |
卷号 | 530期号:40180页码:75-81 |
ISSN号 | 0022-0728 |
关键词 | SITE-DIRECTED MUTAGENESIS CYTOCHROME-C ELECTRON-TRANSFER DIRECT ELECTROCHEMISTRY CONFORMATIONAL-CHANGES PROTEINS LIGAND HISTIDINE REDUCTION PHENYLALANINE-82 |
通讯作者 | zheng jw |
中文摘要 | the structural stability and redox properties of yeast iso-1-cytochrome c and its mutant, f82h, were studied by surface-enhanced resonance raman scattering (serrs) spectroscopy. phenylalanine, which exists at the position-82 in yeast iso-1-cytochrome c, is replaced by histidine in the mutant. the serrs spectra of the proteins on the bare silver electrodes indicate that the mutant possesses a more stable global structure with regard to the adsorption-induced conformational alteration. the redox potential of the mutant negatively shifts by about 400 mv, relative to that of yeast iso-1-cytochrome c. this is ascribed to axial ligand switching and higher solvent accessibility of the heme iron in the mutant during the redox reactions. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000177594900011 |
公开日期 | 2010-11-03 |
源URL | [http://202.98.16.49/handle/322003/18369] |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Zheng JW,Zhou Q,Zhou YG,et al. Surface-enhanced resonance Raman spectroscopic study of yeast iso-1-cytochrome c and its mutant[J]. journal of electroanalytical chemistry,2002,530(40180):75-81. |
APA | Zheng JW,Zhou Q,Zhou YG,Lu TH,Cotton TM,&Chumanov G.(2002).Surface-enhanced resonance Raman spectroscopic study of yeast iso-1-cytochrome c and its mutant.journal of electroanalytical chemistry,530(40180),75-81. |
MLA | Zheng JW,et al."Surface-enhanced resonance Raman spectroscopic study of yeast iso-1-cytochrome c and its mutant".journal of electroanalytical chemistry 530.40180(2002):75-81. |
入库方式: OAI收割
来源:长春应用化学研究所
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