Conformational transition of DNA induced by cationic lipid vesicle in acidic solution: spectroscopy investigation
文献类型：期刊论文
| 作者 | Zhang ZL ; Huang WM ; Tang JL ; Wang EK ; Dong SJ |
| 刊名 | biophysical chemistry
 |
| 出版日期 | 2002 |
| 卷号 | 97期号:1页码:7-16 |
| 关键词 | CALF THYMUS DNA LIPOSOME COMPLEXES GENE-TRANSFER B-DNA SPECTROELECTROCHEMISTRY CONDENSATION ASSEMBLIES MEMBRANES BILAYERS BEHAVIOR |
| ISSN号 | 0301-4622 |
| 通讯作者 | wang ek |
| 中文摘要 | the conformational transition of dna induced by the interaction between dna and a cationic lipid vesicle, didodecyidimethylammonium bromide (ddab), had been investigated by circular dichroism (cd) and uv spectroscopy methods. we used singular value decomposition least squares method (svdls) to analyze the experimental cd spectra. although ph value influenced the conformation of dna in solution, the results showed that upon binding to double helical dna, positively charged liposomes induced a conformational transition of dna molecules from the native b-form to more compact conformations. at the same time, no obvious conformational changes occurred at single-strand dna (ssdna). while the cationic lipid vesicles and double-strand dna (dsdna) were mixed at a high molar ratio of ddab vesicles to dsdna, the conformation of dsdna transformed from the b-form to the c-form resulting in an increase in duplex stability (deltat(m) = 8 +/- 0.4 degreesc). an increasing in t-m was also observed while the cationic lipid vesicles interacted with ssdna. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000176292600002 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18517]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang ZL,Huang WM,Tang JL,et al. Conformational transition of DNA induced by cationic lipid vesicle in acidic solution: spectroscopy investigation[J]. biophysical chemistry,2002,97(1):7-16. |
| APA | Zhang ZL,Huang WM,Tang JL,Wang EK,&Dong SJ.(2002).Conformational transition of DNA induced by cationic lipid vesicle in acidic solution: spectroscopy investigation.biophysical chemistry,97(1),7-16. |
| MLA | Zhang ZL,et al."Conformational transition of DNA induced by cationic lipid vesicle in acidic solution: spectroscopy investigation".biophysical chemistry 97.1(2002):7-16. |
入库方式： OAI收割
来源：长春应用化学研究所
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