Structural electrochemical study of hemoglobin by in situ circular dichroism thin layer spectroelectrochemistry
文献类型：期刊论文
| 作者 | Zhu YC ; Cheng GJ ; Dong SJ |
| 刊名 | biophysical chemistry
 |
| 出版日期 | 2002 |
| 卷号 | 97期号:2-3页码:129-138 |
| 关键词 | SPECTROSCOPY MECHANISMS ELECTRODE |
| ISSN号 | 0301-4622 |
| 通讯作者 | dong sj |
| 中文摘要 | secondary and tertiary or quaternary structural changes in hemoglobin (hb) during an electroreduction process were studied by in situ circular dichroism (cd) spectroelectrochemistry with a long optical path thin-layer cell. by means of singular value decomposition least-squares analysis, cd spectra in the far-uv region give two similar a components with different cd intensity, indicating slight denaturation in the secondary structures due to the electric field effect. cd spectra in the soret band show a r --> t transition of two quaternary structural components induced by electroreduction of the heme, which changes the redox states of the center ion from fe3+ to fe2+ and the coordination number from 6 to 5. the double logarithmic analysis shows that electroreduction of hemoglobin follows a chemical reaction with r --> t transition. some parameters in the electrochemical process were obtained: formal potential, e-0t = -0.167 v; electrochemical kinetic overpotential, deltae(0) = -0.32 v; standard electrochemical reaction rate constant, k(0) = 1.79 x 10(-5) cm s(-1); product of electron transfer coefficient and electron number, alphan=0.14; and the equilibrium constant of r --> t transition, k-c = 9.0. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000176292700004 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18519]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhu YC,Cheng GJ,Dong SJ. Structural electrochemical study of hemoglobin by in situ circular dichroism thin layer spectroelectrochemistry[J]. biophysical chemistry,2002,97(2-3):129-138. |
| APA | Zhu YC,Cheng GJ,&Dong SJ.(2002).Structural electrochemical study of hemoglobin by in situ circular dichroism thin layer spectroelectrochemistry.biophysical chemistry,97(2-3),129-138. |
| MLA | Zhu YC,et al."Structural electrochemical study of hemoglobin by in situ circular dichroism thin layer spectroelectrochemistry".biophysical chemistry 97.2-3(2002):129-138. |
入库方式： OAI收割
来源：长春应用化学研究所
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