Amplified immunoassay of human IgG using real-time biomolecular interaction analysis (BIA) technology
文献类型：期刊论文
| 作者 | Pei RJ ; Cui XQ ; Yang XR ; Wang EK |
| 刊名 | chinese journal of chemistry
 |
| 出版日期 | 2002 |
| 卷号 | 20期号:5页码:441-446 |
| ISSN号 | 1001-604x |
| 关键词 | SURFACE-PLASMON RESONANCE BIOSPECIFIC INTERACTION ANALYSIS PROTEIN BIOSENSOR IMMOBILIZATION COMPLEX BINDING SENSORS MATRIX FILMS |
| 通讯作者 | yang xr |
| 中文摘要 | an automated biomolecular interaction analysis instrument (bi-acore) based on surface plasmon resonance (spr) has been used to determine human immunoglobulin g (igg) in real time. polyclonal anti-human igg antibody was covalently immobilized to a carboxymethyldextran modified gold film surface. the samples of human igg prepared in hbs buffer were poured over the immobilized surface. the signal amplification antibody was applied to amplify the response signal. after each measurement, the surface was regenerated with 0.1 mol/l h3po4. the assay was rapid, requiring only 30 min for antibody immobilization and 20 min for each subsequent process of immune binding, antibody amplification and regeneration. the antibody immobilized surface had good response to human igg in the range of 0.12-60 nmol/l with a detection limit of 60 pmol/l. the same antibody immobilized surface could be used for more than 110 cycles of binding, amplification and regeneration. the results demonstrate that the sensitivity, specificity and reproducibility of amplified immunoassay using real-time bia technology are satisfactory. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000175713800006 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18603]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pei RJ,Cui XQ,Yang XR,et al. Amplified immunoassay of human IgG using real-time biomolecular interaction analysis (BIA) technology[J]. chinese journal of chemistry,2002,20(5):441-446. |
| APA | Pei RJ,Cui XQ,Yang XR,&Wang EK.(2002).Amplified immunoassay of human IgG using real-time biomolecular interaction analysis (BIA) technology.chinese journal of chemistry,20(5),441-446. |
| MLA | Pei RJ,et al."Amplified immunoassay of human IgG using real-time biomolecular interaction analysis (BIA) technology".chinese journal of chemistry 20.5(2002):441-446. |
入库方式： OAI收割
来源：长春应用化学研究所
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