Kinetic analysis of TNF immune complex by using surface plasmon resonane
文献类型：期刊论文
| 作者 | Yang F ; Cui XQ ; Yang XR ; Chen T ; Lu J |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2002 |
| 卷号 | 23期号:3页码:382-384 |
| 关键词 | BIOSPECIFIC INTERACTION ANALYSIS BIOSENSOR IMMOBILIZATION PROTEINS BINDING SERUM |
| ISSN号 | 0251-0790 |
| 通讯作者 | yang xr |
| 中文摘要 | the kinetic analysis of the interaction between tumor necrosis factor(tnf) and its monoclonal antibody was performed by surface plasmon resonance(spr) technique. the monoclonal antibody was immobilized to the surface of cm5 sensor chip by amine coupling. tnf at different concentrations was injected across the mab immobilized surface. the interaction was recorded in real time and could be seen on the sensorgram. one cycle, including association, dissociation and regeneration, lasted no more than 15 min. the interaction results was evaluated using 1 : 1 langmuir binding model. the kinetic rate constants were calculated to be: k =1.68 x 10(3) l (.) mol(-1) (.) s(-1), k(d) = 1.73 x 10(-4) s(-1), and the affinity constants k-a = 9. 7 x 10(3) l (.) mol(-1), k-r)= 1. 03 x 10(-7) mol (.) l-1. the x-2 was 3.47, which showed that the interaction is consistent with the 1 : i model. we can see from the results that although there are two binding sites in one mab molecule, tnf reacts with each site in an independent and noncooperative manner. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000175465700011 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18623]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang F,Cui XQ,Yang XR,et al. Kinetic analysis of TNF immune complex by using surface plasmon resonane[J]. chemical journal of chinese universities-chinese,2002,23(3):382-384. |
| APA | Yang F,Cui XQ,Yang XR,Chen T,&Lu J.(2002).Kinetic analysis of TNF immune complex by using surface plasmon resonane.chemical journal of chinese universities-chinese,23(3),382-384. |
| MLA | Yang F,et al."Kinetic analysis of TNF immune complex by using surface plasmon resonane".chemical journal of chinese universities-chinese 23.3(2002):382-384. |
入库方式： OAI收割
来源：长春应用化学研究所
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