Layer-by-layer assembly of multilayer films composed of streptavidin and biotinylated antibody by real-time biomolecular interaction analysis
文献类型：期刊论文
| 作者 | Pei RJ ; Cui XQ ; Yang XR ; Wang EK |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2002 |
| 卷号 | 23期号:2页码:195-198 |
| 关键词 | BIOSPECIFIC INTERACTION ANALYSIS 3-DIMENSIONAL STRUCTURE THIN-FILMS AVIDIN CONSTRUCTION RESOLUTION PROTEINS SURFACES COMPLEX SENSORS |
| ISSN号 | 0251-0790 |
| 通讯作者 | yang xr |
| 中文摘要 | layer-by-layer assembly of multilayer films of streptavidin and biotinylated antibody was completed on the streptavidin coated surface. real-time biomolecular interaction analysis (bia) based on surface plasmon resonance technique was used to monitor the multilayer assembly in solution continuously. the results indicate that the uniform multilayer film can be fabricated successfully based on the strong interaction between streptavidin and biotin. the mean surface mass concentration of each adsorption layer is 1. 32 ng/mm(2) for biotinylated antibody, 2. 93 ng/mm(2) for streptavidin, according to the correlation of spr response with surface concentration. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000174163300008 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18751]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pei RJ,Cui XQ,Yang XR,et al. Layer-by-layer assembly of multilayer films composed of streptavidin and biotinylated antibody by real-time biomolecular interaction analysis[J]. chemical journal of chinese universities-chinese,2002,23(2):195-198. |
| APA | Pei RJ,Cui XQ,Yang XR,&Wang EK.(2002).Layer-by-layer assembly of multilayer films composed of streptavidin and biotinylated antibody by real-time biomolecular interaction analysis.chemical journal of chinese universities-chinese,23(2),195-198. |
| MLA | Pei RJ,et al."Layer-by-layer assembly of multilayer films composed of streptavidin and biotinylated antibody by real-time biomolecular interaction analysis".chemical journal of chinese universities-chinese 23.2(2002):195-198. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。