Enhanced surface plasmon resonance immunoassay for human complement factor 4
文献类型：期刊论文
| 作者 | Pei RJ ; Yang XR ; Wang EK |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2002 |
| 卷号 | 453期号:2页码:173-179 |
| 关键词 | BIOSPECIFIC INTERACTION ANALYSIS PROTEIN ANTIBODY IMMOBILIZATION BIOSENSOR BINDING SERUM C5A |
| ISSN号 | 0003-2670 |
| 通讯作者 | wang ek |
| 中文摘要 | using an enhanced surface plasmon resonance (spr) immunosensor, we have determined the concentration of human complement factor 4 (c4). antibody protein was concentrated into a carboxymethyldextran-modified gold surface by electrostatic attraction force and a simultaneous covalent immobilization of antibody based on amine coupling reaction took place. the sandwich method was applied to enhance the response signal and the specificity of antigen binding assay. the antibody immobilized surface had good response to c4 in the range of 0.02-20 mug/ml by this enhanced immunoassay. the regeneration effect by ph 2 glycine-hc1 buffer was also investigated. the same antibody immobilized surface could be used more than 80 cycles of c4 binding and regeneration. in addition, the ability to determinate c4 directly from serum sample without any purification was investigated. the sensitivity, specificity and reproducibility of the enhanced immunoassay are satisfactory. the results clearly demonstrate the advantages of the enhanced spr technique for c4 immunoassay. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000173946300002 |
| 公开日期 | 2010-11-03 |
| 源URL | [http://202.98.16.49/handle/322003/18775]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pei RJ,Yang XR,Wang EK. Enhanced surface plasmon resonance immunoassay for human complement factor 4[J]. analytica chimica acta,2002,453(2):173-179. |
| APA | Pei RJ,Yang XR,&Wang EK.(2002).Enhanced surface plasmon resonance immunoassay for human complement factor 4.analytica chimica acta,453(2),173-179. |
| MLA | Pei RJ,et al."Enhanced surface plasmon resonance immunoassay for human complement factor 4".analytica chimica acta 453.2(2002):173-179. |
入库方式： OAI收割
来源：长春应用化学研究所
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