Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor
文献类型：期刊论文
| 作者 | Pei RJ ; Cui XQ ; Yang XR ; Wang EK |
| 刊名 | talanta
 |
| 出版日期 | 2000 |
| 卷号 | 53期号:3页码:481-488 |
| 关键词 | BIOSPECIFIC INTERACTION ANALYSIS COMPLEX IMMOBILIZATION AFFINITY PROTEINS SENSORS BINDING LIGANDS FILMS HIV-1 |
| ISSN号 | 0039-9140 |
| 通讯作者 | yang xr |
| 中文摘要 | a surface plasmon resonance biosensor has been used to determine antibody activity in serum. as a model system, the interaction of mouse igg and sheep anti-mouse igg polyclonal antibody was investigated in real time. the factors, including ph value, ionic strength, protein concentration, influencing electrostatic adsorption of mouse igg protein onto carboxylated dextran-coated sensor chip surface, were studied. the procedures of mouse igg protein immobilization and immune reaction were monitored in real time. the regeneration effect using the different elution reagents was also investigated. the same mouse igg immobilized surface can be used for 100 cycles of binding and elution with only 0.38% loss per regeneration in reactivity. the results show that the surface plasmon resonance biosensor is a rapid, simple, sensitive, accurate and reliable detection technique for real-time immunoassay of antibody activity. the assay allows antibodies to be detected and studied in their native form without any purification. (c) 2000 elsevier science b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000165524200001 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/19449]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pei RJ,Cui XQ,Yang XR,et al. Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor[J]. talanta,2000,53(3):481-488. |
| APA | Pei RJ,Cui XQ,Yang XR,&Wang EK.(2000).Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor.talanta,53(3),481-488. |
| MLA | Pei RJ,et al."Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor".talanta 53.3(2000):481-488. |
入库方式： OAI收割
来源：长春应用化学研究所
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