Organic-phase enzyme electrode for phenolic determination based on a functionalized sol-gel composite
文献类型:期刊论文
作者 | Wang BQ ; Dong SJ |
刊名 | journal of electroanalytical chemistry
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出版日期 | 2000 |
卷号 | 487期号:1页码:45-50 |
关键词 | TYROSINASE-BASED BIOSENSOR AMPEROMETRIC BIOSENSOR HYDROGEN-PEROXIDE POLY(VINYL ALCOHOL) GLUCOSE BIOSENSOR CONSTRUCTION SOLVENTS WATER MEDIA COPOLYMER |
ISSN号 | 0022-0728 |
通讯作者 | dong sj |
中文摘要 | an amperometric biosensor for monitoring phenols in the organic phase was constructed by the silica sol-gel immobilization of tyrosinase on a glassy carbon electrode. the organic-inorganic hybrid materials with different sol-gel precursors and polymers were optimized, and the experimental conditions, such as the effect of the solvent, operational potential and enzyme loading were explored for the optimum analytical performance of the enzyme electrode. the biosensor can reach 95% of steady-state current in about 18 s, and the trend in the sensitivity of different phenols is as follows: catechol > phenol >p-cresol. in addition, the apparent michaelis-menten constants (k-m(app)) and the stability of the enzyme electrode were discussed. (c) 2000 elsevier science s.a. all rights reserved. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000165580200006 |
公开日期 | 2010-11-04 |
源URL | [http://202.98.16.49/handle/322003/19453] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Wang BQ,Dong SJ. Organic-phase enzyme electrode for phenolic determination based on a functionalized sol-gel composite[J]. journal of electroanalytical chemistry,2000,487(1):45-50. |
APA | Wang BQ,&Dong SJ.(2000).Organic-phase enzyme electrode for phenolic determination based on a functionalized sol-gel composite.journal of electroanalytical chemistry,487(1),45-50. |
MLA | Wang BQ,et al."Organic-phase enzyme electrode for phenolic determination based on a functionalized sol-gel composite".journal of electroanalytical chemistry 487.1(2000):45-50. |
入库方式: OAI收割
来源:长春应用化学研究所
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