Laser-time-resolved fluorescence spectroscopy in immunoassays
文献类型：期刊论文
| 作者 | Pan LH ; Du JX ; Xie WB ; Du QY ; Yun Q |
| 刊名 | spectroscopy and spectral analysis
 |
| 出版日期 | 2000 |
| 卷号 | 20期号:3页码:277-279 |
| ISSN号 | 1000-0593 |
| 通讯作者 | pan lh |
| 中文摘要 | this paper described a laser-excited time-resolved fluoroimmunoassay set. it made lanthanide ion to couple the anhydrde of diethylenetriaminepentaacetic acid (dtpaa) for labeling antibodies. the experiment used polystyrene tap coated with hcv antigen as the solid phase and a chelate of the rare earth metal europium as fluorescent label. a nitrogen laser beam was used to excite the eu3+ chelates and after 60 ys delay time,the emission fluorescence was measured. background fluorescence of short lifetimes caused by serum components and raman scattering can be eliminated by set the delay rime. in the system condition, fluorescent spectra and fluorescent lifetimes of eu3+ beta-naphthoyltrifluroacetone (nta) chelates were measured. the fluorescent lifetime value is 650 mu s. the maximum emssion wavelength is 613 nm. the linear range of europium ion concentration is 1 x 10(-7)- 1 x 10(-11) g.ml(-1) and the detection limit is 1 x 10(-13) g.ml(-1). the relative standard deviation of determination ( n = 12) for samples at 0.01 ng.ml(-1) magnitude is 6.4%. laser-trfia was also found to be suitable for diagnosis of hcv. the sensitvity and specificity were comparable to enzyme immunoassay. the result was obtained with laser-trfia for 29 human correlated well with enzyme immunoassay. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000088966900007 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/19619]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pan LH,Du JX,Xie WB,et al. Laser-time-resolved fluorescence spectroscopy in immunoassays[J]. spectroscopy and spectral analysis,2000,20(3):277-279. |
| APA | Pan LH,Du JX,Xie WB,Du QY,&Yun Q.(2000).Laser-time-resolved fluorescence spectroscopy in immunoassays.spectroscopy and spectral analysis,20(3),277-279. |
| MLA | Pan LH,et al."Laser-time-resolved fluorescence spectroscopy in immunoassays".spectroscopy and spectral analysis 20.3(2000):277-279. |
入库方式： OAI收割
来源：长春应用化学研究所
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