Circular dichroism and resonance Raman comparative studies of wild type cytochrome c and F82H mutant
文献类型：期刊论文
| 作者 | Zheng JW ; Ye SY ; Lu TH ; Cotton TM ; Chumanov G |
| 刊名 | biopolymers
 |
| 出版日期 | 2000 |
| 卷号 | 57期号:2页码:77-84 |
| ISSN号 | 0006-3525 |
| 关键词 | SITE-DIRECTED MUTAGENESIS YEAST ISO-1-CYTOCHROME-C CONFORMATIONAL-CHANGES ELECTRON-TRANSFER LIGAND PHENYLALANINE-82 POSITION-82 REPLACEMENT ASSIGNMENT HISTIDINE |
| 通讯作者 | chumanov g |
| 中文摘要 | the uv-visible, circular dichroism (cd), and resonance raman (rr) spectra of the wild type yeast iso-1-cytochrome c (wt) and its mutant f82h in which phenylalanine-82 (phe-82) is substituted with his are measured and compared for oxidized and reduced forms. the cd spectra in the intrinsic and soret spectral region, as well as rr spectra in high, middle, and low frequency regions, are discussed. from the analysis of the spectra, it is determined that in the oxidized f82h the two axial ligands to the heme iron are his-18 and his-82 whereas in the reduced form the sixth ligand switches from his-82 to met-80 providing the coordination geometry similar to that of wt. based on the spectroscopic data, the conclusion is that the porphyrin macrocycle is less distorted in the oxidized f82h compared to the oxidized wt. similar distortions are present in the reduced form of the proteins. frequency shifts of raman bands, as well as the decrease of the or-helix content in the cd spectra, indicate more open conformation of the protein around the heme. (c) 2000 john wiley & sons, inc. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000086468200004 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/19793]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zheng JW,Ye SY,Lu TH,et al. Circular dichroism and resonance Raman comparative studies of wild type cytochrome c and F82H mutant[J]. biopolymers,2000,57(2):77-84. |
| APA | Zheng JW,Ye SY,Lu TH,Cotton TM,&Chumanov G.(2000).Circular dichroism and resonance Raman comparative studies of wild type cytochrome c and F82H mutant.biopolymers,57(2),77-84. |
| MLA | Zheng JW,et al."Circular dichroism and resonance Raman comparative studies of wild type cytochrome c and F82H mutant".biopolymers 57.2(2000):77-84. |
入库方式： OAI收割
来源：长春应用化学研究所
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