Studies of soluble human catalytic antibody with glutathione peroxidase activity
文献类型：期刊论文
| 作者 | Qi XJ ; Wang L ; Fang F ; Yan XY ; Ding L ; Zhao DQ ; Ni JZ |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:12页码:1976-1978 |
| 关键词 | SELENOCYSTEINE SELENIUM |
| ISSN号 | 0251-0790 |
| 通讯作者 | qi xj |
| 中文摘要 | by screening the phage-displayed human single chain antibody library, we have got the specific single chain antibody bound to gsh-s-dnp butyl ester as the hapten. the tertiary structure of the protein was analyzed with the aid of computer, and the results showed the cdr3 region located on the surface of the antibody. the soluble antibody was expressed in e. coli. and the active site serine was converted into selenocysteine with the chemical modifying method, which resulted in the catalytic antibody with gpx activity of 80 u/mu mol. furthermore, the same ping-pong mechanism as the natural gpx was observed when the kinetic behavior of the antibody was studied. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000172815300004 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20429]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qi XJ,Wang L,Fang F,et al. Studies of soluble human catalytic antibody with glutathione peroxidase activity[J]. chemical journal of chinese universities-chinese,2001,22(12):1976-1978. |
| APA | Qi XJ.,Wang L.,Fang F.,Yan XY.,Ding L.,...&Ni JZ.(2001).Studies of soluble human catalytic antibody with glutathione peroxidase activity.chemical journal of chinese universities-chinese,22(12),1976-1978. |
| MLA | Qi XJ,et al."Studies of soluble human catalytic antibody with glutathione peroxidase activity".chemical journal of chinese universities-chinese 22.12(2001):1976-1978. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。