Use of nitrocellulose films for affinity-directed mass spectrometry for the analysis of antibody/antigen interactions
文献类型：期刊论文
| 作者 | Sun SQ ; Mo WJ ; Ji YP ; Liu SY |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2001 |
| 卷号 | 15期号:18页码:1743-1746 |
| 关键词 | PROTEIN IDENTIFICATION EPITOPES |
| ISSN号 | 0951-4198 |
| 通讯作者 | liu sy |
| 中文摘要 | combination of affinity extraction procedures with mass spectrometric analyses is termed affinity-directed mass spectrometry, a technique that has gained broad interest in immunology and is extended here with several improvements from methods used in previous studies. a monoclonal antibody was immobilized on a nitrocellulose (nc) membrane, allowing the corresponding antigen to be selectively captured from a complex solution for analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms). this method was also used to rapidly determine the approximate binding region responsible for the antibody/antigen interaction. the tryptic fragments of antigen protein in buffer were applied to the antibody immobilized on nc film and allowed to interact. the nc film was then washed to remove salts and other unbound components, and subjected to analysis by maldi-tofms. using interferon-alpha (2a) and anti-interferon-alpha (2a) monoclonal antibody igg as a model system, we successfully extracted the antigen protein and determined the approximate binding region for the antigen/antibody interaction (i.e., the tryptic fragment responsible). copyright (c) 2001 john wiley & sons, ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000171017900013 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20561]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun SQ,Mo WJ,Ji YP,et al. Use of nitrocellulose films for affinity-directed mass spectrometry for the analysis of antibody/antigen interactions[J]. rapid communications in mass spectrometry,2001,15(18):1743-1746. |
| APA | Sun SQ,Mo WJ,Ji YP,&Liu SY.(2001).Use of nitrocellulose films for affinity-directed mass spectrometry for the analysis of antibody/antigen interactions.rapid communications in mass spectrometry,15(18),1743-1746. |
| MLA | Sun SQ,et al."Use of nitrocellulose films for affinity-directed mass spectrometry for the analysis of antibody/antigen interactions".rapid communications in mass spectrometry 15.18(2001):1743-1746. |
入库方式： OAI收割
来源：长春应用化学研究所
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