Amplification of antigen-antibody interactions based on biotin labeled protein-streptavidin network complex using impedance spectroscopy
文献类型：期刊论文
| 作者 | Pei RJ ; Cheng ZL ; Wang EK ; Yang XR |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2001 |
| 卷号 | 16期号:6页码:355-361 |
| 关键词 | QUARTZ-CRYSTAL MICROBALANCE SELF-ASSEMBLED MONOLAYERS INSOLUBLE PRODUCT IMPEDIMETRIC IMMUNOSENSOR ELECTRODES PRECIPITATION FILMS SUPPORTS BEHAVIOR |
| ISSN号 | 0956-5663 |
| 通讯作者 | yang xr |
| 中文摘要 | antibody was covalently immobilized by amine coupling method to gold surfaces modified with a self-assembled monolayer of thioctic acid. the electrochemical measurements of cyclic voltammetry and impedance spectroscopy showed that the hexacyanoferrate redox reactions on the gold surface were blocked due to the procedures of self-assembly of thioctic acid and antibody immobilization. the binding of a specific antigen to antibody recognition layer could be detected by measurements of the impedance change. a new amplification strategy was introduced for improving the sensitivity of impedance measurements using biotin labeled protein- streptavidin network complex. this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. this complex can be formed in a cross-linking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex. the results show that this amplification strategy causes dramatic improvement of the detection sensitivity of higg and has good correlation for detection of higg in the range of 2-10 mug/ml. (c) 2001 elsevier science b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000170722800002 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20611]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pei RJ,Cheng ZL,Wang EK,et al. Amplification of antigen-antibody interactions based on biotin labeled protein-streptavidin network complex using impedance spectroscopy[J]. biosensors & bioelectronics,2001,16(6):355-361. |
| APA | Pei RJ,Cheng ZL,Wang EK,&Yang XR.(2001).Amplification of antigen-antibody interactions based on biotin labeled protein-streptavidin network complex using impedance spectroscopy.biosensors & bioelectronics,16(6),355-361. |
| MLA | Pei RJ,et al."Amplification of antigen-antibody interactions based on biotin labeled protein-streptavidin network complex using impedance spectroscopy".biosensors & bioelectronics 16.6(2001):355-361. |
入库方式： OAI收割
来源：长春应用化学研究所
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