Human catalytic antibodies with glutathione peroxidase activity
文献类型：期刊论文
| 作者 | Fang F ; Wang L ; Qi XJ ; Yan XY ; Ding L ; Zhao DQ ; Ni JZ |
| 刊名 | journal of inorganic biochemistry
 |
| 出版日期 | 2001 |
| 卷号 | 85期号:4页码:301-307 |
| 关键词 | PHAGE DISPLAY LIBRARY SELENIUM SELENOCYSTEINE CONVERSION SEQUENCES SEGMENTS BINDING THERAPY ABZYME |
| ISSN号 | 0162-0134 |
| 通讯作者 | ding l |
| 中文摘要 | in order to generate catalytic antibodies with glutathione peroxidase (gpx) activity, we prepared gsh-s-dnp butyl ester and gsh-s-dnp benzyl ester as the haptens. two scfvs that bound specifically to the haptens were selected from the human phage-displayed antibody library. the two scfv genes were highly homologous, consisting of 786 bps and belonging to the same vh family-dp25. in the premise of maintaining the amino acid sequence, mutated plasmids were constructed by use of the mutated primers in pcr, and they were over-expressed in e. coli. after the active site serine was converted into selenocysteine with the chemical modifying method, we obtained two human catalytic antibodies with gpx activity of 72.2u/mu mol and 28.8u/mu mol, respectively. with the aid of computer mimicking, it can be assumed that the antibodies can form dimers and the mutated selenocysteine residue is located in the binding site. furthermore, the same ping-pong mechanism as the natural gpx was observed when the kinetic behavior of the antibody with the higher activity was studied. (c) 2001 elsevier science by. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000170339900008 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20661]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Fang F,Wang L,Qi XJ,et al. Human catalytic antibodies with glutathione peroxidase activity[J]. journal of inorganic biochemistry,2001,85(4):301-307. |
| APA | Fang F.,Wang L.,Qi XJ.,Yan XY.,Ding L.,...&Ni JZ.(2001).Human catalytic antibodies with glutathione peroxidase activity.journal of inorganic biochemistry,85(4),301-307. |
| MLA | Fang F,et al."Human catalytic antibodies with glutathione peroxidase activity".journal of inorganic biochemistry 85.4(2001):301-307. |
入库方式： OAI收割
来源：长春应用化学研究所
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