Preparation and properties of a selenium-containing catalytic antibody as type I deiodinase mimic
文献类型：期刊论文
| 作者 | Lian GW ; Ding L ; Chen M ; Liu Z ; Zhao DQ ; Ni JZ |
| 刊名 | journal of biological chemistry
 |
| 出版日期 | 2001 |
| 卷号 | 276期号:30页码:28037-28041 |
| 关键词 | RAT-LIVER IODOTHYRONINE DEIODINASE PARTIAL-PURIFICATION 5'-DEIODINASE MICROSOMES THYROXINE ENZYME KIDNEY |
| ISSN号 | 0021-9258 |
| 通讯作者 | ding l |
| 中文摘要 | conversion of thyroxine (t-4) to 3,5,3'-triiodothyronine is an essential first step in controlling thyroid hormone action. type i deiodinase (di) can catalyze the conversion to produce the bulk of serum 3,5,3'-triiodothyronine. acting as a mimic of di, a selenium-containing catalytic antibody (se-4c5) prepared by converting the serine residues of monoclonal antibody 4c5 raised against t4 into selenocysteines, can catalyze the deiodination of t4 with dithiothreitol (dtt) as cosubstrate. the mimic enzyme se-4c5 exhibited a much greater deiodinase activity than model compound ebselen and another selenium-containing antibody se-hp4 against gsh. the coupling of selenocysteine with the combining pocket of antibody 4c5 endowed se-4c5 with enzymatic activity. to probe the catalytic mechanism of the catalytic antibody, detailed kinetic studies were carried out in this paper. investigations into the deiodinative reaction revealed the relationship between the initial velocity and substrate concentration. the characteristic parallel dalziel plots demonstrated that se-4c5-catalyzed reaction mechanism was ping-pong one, involving at least one covalent enzyme intermediate. the kinetic properties of the catalytic antibody were similar to those of di, with k-m values for t-4 and dtt of approximately 0.8 mum and 1.8 mum, respectively, and a v-m value of 270 pmol per mg of protein per min. the activity could be sensitively inhibited by 6-propyl-2-thiouracil (ptu) with a k-i value of similar to 120 mum at 2.0 mum t-4 concentration. the ptu inhibition was progressively alleviated with the increasing concentration of added dtt, revealing that ptu was a competitive inhibitor for dtt. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000170093400040 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20675]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lian GW,Ding L,Chen M,et al. Preparation and properties of a selenium-containing catalytic antibody as type I deiodinase mimic[J]. journal of biological chemistry,2001,276(30):28037-28041. |
| APA | Lian GW,Ding L,Chen M,Liu Z,Zhao DQ,&Ni JZ.(2001).Preparation and properties of a selenium-containing catalytic antibody as type I deiodinase mimic.journal of biological chemistry,276(30),28037-28041. |
| MLA | Lian GW,et al."Preparation and properties of a selenium-containing catalytic antibody as type I deiodinase mimic".journal of biological chemistry 276.30(2001):28037-28041. |
入库方式： OAI收割
来源：长春应用化学研究所
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