A selenium-containing catalytic antibody with type I deiodinase activity
文献类型：期刊论文
| 作者 | Lian GW ; Ding L ; Chen M ; Liu LY ; Zhao D ; Ni J |
| 刊名 | biochemical and biophysical research communications
 |
| 出版日期 | 2001 |
| 卷号 | 283期号:5页码:1007-1012 |
| ISSN号 | 0006-291x |
| 关键词 | RAT-LIVER IODOTHYRONINE DEIODINASE PARTIAL-PURIFICATION 5'-DEIODINASE MICROSOMES THYROXINE ENZYME KIDNEY |
| 通讯作者 | ni j |
| 中文摘要 | acting as a mimic of type i deiodinase (di), a selenium-containing catalytic antibody (se-4c5) prepared by converting the serine residues of monoclonal antibody 4c5 raised against thyroxine (t-4) into selenocysteines, can catalyze the deiodination of t-4 to 3,5,3'-triiodothyronine (t-3) with dithiothreitol (dtt) as cosubstrate. investigations into the deiodinative reaction by se-4c5 revealed the relationship between the initial velocity and substrate concentration was subjected to michaelis-menten equation and the reaction mechanism was ping-pong one. the kinetic properties of the catalytic antibody were a little similar to those of di, with k-m values for t-4 and dtt of approximately 0.8 mum and 1.8 mm, respectively, and v-m value of 270 pmol per mg protein per min. the activity could be sensitively inhibited by ptu with a k-i value of approximately 120 mum at 2.0 mum of t-4 concentration, revealing that ptu was a competitive inhibitor for dtt, (c) 2001 academic press. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000169056400002 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20753]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lian GW,Ding L,Chen M,et al. A selenium-containing catalytic antibody with type I deiodinase activity[J]. biochemical and biophysical research communications,2001,283(5):1007-1012. |
| APA | Lian GW,Ding L,Chen M,Liu LY,Zhao D,&Ni J.(2001).A selenium-containing catalytic antibody with type I deiodinase activity.biochemical and biophysical research communications,283(5),1007-1012. |
| MLA | Lian GW,et al."A selenium-containing catalytic antibody with type I deiodinase activity".biochemical and biophysical research communications 283.5(2001):1007-1012. |
入库方式： OAI收割
来源：长春应用化学研究所
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