Acid-stable amperometric soybean peroxidase biosensor based on a self-gelatinizable grafting copolymer of polyvinyl alcohol and 4-vinylpyridine
文献类型：期刊论文
| 作者 | Wang BQ ; Li B ; Cheng GJ ; Dong SJ |
| 刊名 | electroanalysis
 |
| 出版日期 | 2001 |
| 卷号 | 13期号:7页码:555-558 |
| 关键词 | HYDROGEN-PEROXIDE HORSERADISH-PEROXIDASE MODIFIED ELECTRODES ENZYME ELECTRODE MONOLAYER SURFACE FILM |
| ISSN号 | 1040-0397 |
| 通讯作者 | dong sj |
| 中文摘要 | a peroxidase was extracted from chinese soybean seed coat, and its thermostability and acid-stability were characterized. this peroxidase was immobilized into a self-gelatinizable grafting copolymer of polyvinyl alcohol with 4-vinylpyridine(pva-g-pvp) to construct an acid-stable hydrogen peroxide biosensor. the effect of ph was studied for optimum analytical performances by amperometric and spectro-photometric methods, also the k-m(app) and the stability of the soybean peroxidase-based biosensor are discussed. at ph 3.0, the soybean peroxidase maintained its bioactivity and the enzyme electrode had a linear range from 0.01 to 6.2 mm with a detection limit of 1.0 x 10(-7) m. in addition, the main characteristics of different hydrogen peroxide sensors were compared. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000168943200007 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20767]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang BQ,Li B,Cheng GJ,et al. Acid-stable amperometric soybean peroxidase biosensor based on a self-gelatinizable grafting copolymer of polyvinyl alcohol and 4-vinylpyridine[J]. electroanalysis,2001,13(7):555-558. |
| APA | Wang BQ,Li B,Cheng GJ,&Dong SJ.(2001).Acid-stable amperometric soybean peroxidase biosensor based on a self-gelatinizable grafting copolymer of polyvinyl alcohol and 4-vinylpyridine.electroanalysis,13(7),555-558. |
| MLA | Wang BQ,et al."Acid-stable amperometric soybean peroxidase biosensor based on a self-gelatinizable grafting copolymer of polyvinyl alcohol and 4-vinylpyridine".electroanalysis 13.7(2001):555-558. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。