Preparation and mass spectrometric study of egg yolk antibody (IgY) against rabies virus
文献类型：期刊论文
| 作者 | Sun SQ ; Mo WJ ; Ji YP ; Liu SY |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2001 |
| 卷号 | 15期号:9页码:708-712 |
| 关键词 | LASER-DESORPTION CHICKEN ANTIBODIES IMMUNOGLOBULINS TOOL |
| ISSN号 | 0951-4198 |
| 通讯作者 | liu sy |
| 中文摘要 | rabies virus was used as the antigen to immunize laying chickens. anti-rabies virus immunoglobulin y(igy) was isolated from yolks of the eggs laid by these chickens using a two-step salt precipitation and one-step gel filtration protocol. the purified igy was reduced with dithiothreitol, and heavy chains (hc) and light chains (lc) were obtained. in addition, the purified igy was digested with pepsin and the fragment with specific antigen binding properties (fab) was produced. using matrix-assisted laser desorption/ionization mass spectrometry (maldi-tofms), the average molecular weights of igy, hc, lc, and fab were determined as 167 250, 65 105, 18 660, and 45,359 da, respectively. igy has two structural differences compared with mammalian iggs. first, the molecular weight of the heavy chain of igy is larger than that of its mammalian counterpart, while the molecular weight of the light chain of igy is smaller. second, upon pepsin digestion, anti-rabies virus igy is degraded into feb, in contrast to mammalian igg, which has been reported to be degraded into f(ab')(2) under the same conditions. copyright (c) 2001 john wiley & sons, ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000168513500008 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20809]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun SQ,Mo WJ,Ji YP,et al. Preparation and mass spectrometric study of egg yolk antibody (IgY) against rabies virus[J]. rapid communications in mass spectrometry,2001,15(9):708-712. |
| APA | Sun SQ,Mo WJ,Ji YP,&Liu SY.(2001).Preparation and mass spectrometric study of egg yolk antibody (IgY) against rabies virus.rapid communications in mass spectrometry,15(9),708-712. |
| MLA | Sun SQ,et al."Preparation and mass spectrometric study of egg yolk antibody (IgY) against rabies virus".rapid communications in mass spectrometry 15.9(2001):708-712. |
入库方式： OAI收割
来源：长春应用化学研究所
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