A facile approach to immobilize protein for biosensor: self-assembled supported bilayer lipid membranes on glassy carbon electrode
文献类型：期刊论文
| 作者 | Wu ZY ; Wang BQ ; Cheng ZL ; Yang XR ; Dong SJ ; Wang EK |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2001 |
| 卷号 | 16期号:1-2页码:47-52 |
| 关键词 | HYDROGEN-PEROXIDE HORSERADISH-PEROXIDASE CYCLIC VOLTAMMETRY ENZYME IMPEDANCE FILMS BLMS |
| ISSN号 | 0956-5663 |
| 通讯作者 | wang ek |
| 中文摘要 | a kind of solid substrate, glassy carbon (gc) electrode. was selected to support self-assembled lipid layer membranes. on the surface of gc electrode. we made layers of dimyristoylphosphatidylcholine (dmpg, a kind of lipid). from electrochemical impedance experiments. we demonstrated that the lipid layers on the gc electrode were bilayer lipid membranes. we immobilized horseradish peroxidase (hrp) into the supported bilayer lipid membranes (s-blm) to develop a kind of mediator-free biosensor for h2o2. the biosensor exhibited fine electrochemical response, stability and reproducibility due to the presence of the s-blm. as a model of biological membrane, s-blm could supply a biological environment for enzyme and maintain its activity. so s-blm is an ideal choice to immobilize enzyme for constructing the mediator-free biosensor based on gc electrode. (c) 2001 elsevier science b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000167408200007 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/20905]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wu ZY,Wang BQ,Cheng ZL,et al. A facile approach to immobilize protein for biosensor: self-assembled supported bilayer lipid membranes on glassy carbon electrode[J]. biosensors & bioelectronics,2001,16(1-2):47-52. |
| APA | Wu ZY,Wang BQ,Cheng ZL,Yang XR,Dong SJ,&Wang EK.(2001).A facile approach to immobilize protein for biosensor: self-assembled supported bilayer lipid membranes on glassy carbon electrode.biosensors & bioelectronics,16(1-2),47-52. |
| MLA | Wu ZY,et al."A facile approach to immobilize protein for biosensor: self-assembled supported bilayer lipid membranes on glassy carbon electrode".biosensors & bioelectronics 16.1-2(2001):47-52. |
入库方式： OAI收割
来源：长春应用化学研究所
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