Self-gelatinizable graft copolymer of poly(vinyl alcohol) with 4-vinylpyridine as an immobilization matrix for the construction of a tyrosinase-based amperometric biosensor
文献类型：期刊论文
| 作者 | Zhang JZ ; Li B ; Xu GB ; Cheng GJ ; Dong SJ |
| 刊名 | analyst
 |
| 出版日期 | 1999 |
| 卷号 | 124期号:5页码:699-703 |
| 关键词 | PURE ORGANIC-PHASE POLYPHENOL OXIDASE GRAPHITE ELECTRODE ENZYME ELECTRODE INHIBITORS SOLVENTS CATECHOL PHENOLS |
| ISSN号 | 0003-2654 |
| 通讯作者 | dong sj |
| 中文摘要 | a tyrosinase-based amperometric biosensor using a self-gelatinizable graft copolymer of poly(vinyl alcohol) with 4-vinylpyridine (pva-g-pvp) as an immobilization matrix was constructed. the 4-vinylpyridine component of pva-g-pvp enhances the adherence to a glassy carbon electrode surface. the content of 4-vinylpyridine in this immobilization matrix plays a key role in retaining the activity of tyrosinase. a simple, milder method was adopted by simply syringing the copolymer-tyrosinase aqueous solution on to the electrode surface and allowing water to evaporate at 4 degrees c in a refrigerator. several parameters, including copolymer composition; ph, applied potential and enzyme membrane composition, ware optimized. the enzyme membrane composition can be varied to obtain higher sensitivity or a wider linear detection range. the biosensor was used for the determination of phenol, p-cresol and catechol. the biosensor exhibited excellent reproducibility, stability and sensitive response and can be used in flow injection analysis. the biosensor showed an extended linear range in hydrophilic organic solvents and it can be used in monitoring organic reaction processes. the analytical performance demonstrated this immobilization matrix is suitable for the immobilization of tyrosinase. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000080389000012 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/21881]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang JZ,Li B,Xu GB,et al. Self-gelatinizable graft copolymer of poly(vinyl alcohol) with 4-vinylpyridine as an immobilization matrix for the construction of a tyrosinase-based amperometric biosensor[J]. analyst,1999,124(5):699-703. |
| APA | Zhang JZ,Li B,Xu GB,Cheng GJ,&Dong SJ.(1999).Self-gelatinizable graft copolymer of poly(vinyl alcohol) with 4-vinylpyridine as an immobilization matrix for the construction of a tyrosinase-based amperometric biosensor.analyst,124(5),699-703. |
| MLA | Zhang JZ,et al."Self-gelatinizable graft copolymer of poly(vinyl alcohol) with 4-vinylpyridine as an immobilization matrix for the construction of a tyrosinase-based amperometric biosensor".analyst 124.5(1999):699-703. |
入库方式： OAI收割
来源：长春应用化学研究所
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