Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom
文献类型：期刊论文
| 作者 | Sun MZ ; Ding L ; Ji YP ; Zhao DQ ; Liu SY ; Ni JZ |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 1999 |
| 卷号 | 13期号:3页码:150-155 |
| 关键词 | WESTERN DIAMONDBACK RATTLESNAKE SIZE-EXCLUSION CHROMATOGRAPHY CONTORTRIX-CONTORTRIX CROTALUS-ATROX DESORPTION SNAKE RESOLUTION ACID |
| ISSN号 | 0951-4198 |
| 通讯作者 | zhao, dq, chinese acad sci, changchun inst appl chem, key lab rare earth chem & phys, changchun 130022, peoples r china |
| 中文摘要 | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms) was used to analyze two enzymes, phospholipase az and fibrinolytic enzyme isolated from chinese agkistrodon blomhoffii ussurensis venom. using sinapinic acid as the matrix, positive ion mass spectra of the enzymes were obtained, in addition to the dominant protein [m+h](+) ions, multimeric and multiply charged ions were also observed in the mass spectra, the higher the concentration of the enzymes, the more multiply charged polymer and multimeric ions were detected, our results indicate that maldi-tofms can provide a rapid and accurate method for molecular weight determination of snake venom enzymes, mass accuracies of 0.1 and 0.3 % were achieved by analysis of highly dialyzed phospholipase a2 and fibrinolytic enzyme, and these results are much better than those obtained using sodium dodecyl sulfate-palyacrylamide gel electrophoresis. maldi-tofms thus provides a reliable method to determine the purity and molecular weight of these enzymes, which are of potential use as therapeutants, copyright (c) 1999 john wiley & sons, ltd. |
| 收录类别 | CSCD收录国内期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000078534100002 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/22027]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun MZ,Ding L,Ji YP,et al. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom[J]. rapid communications in mass spectrometry,1999,13(3):150-155. |
| APA | Sun MZ,Ding L,Ji YP,Zhao DQ,Liu SY,&Ni JZ.(1999).Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom.rapid communications in mass spectrometry,13(3),150-155. |
| MLA | Sun MZ,et al."Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom".rapid communications in mass spectrometry 13.3(1999):150-155. |
入库方式： OAI收割
来源：长春应用化学研究所
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