Detection of specific noncovalent protein-fullerenols complexes by matrix-assisted laser desorption ionization mass spectrometry
文献类型：期刊论文
| 作者 | She YM ; Ji YP ; He YF ; Liu SY |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 1998 |
| 卷号 | 19期号:11页码:1735-1738 |
| 关键词 | DERIVATIVES INHIBITION |
| ISSN号 | 0251-0790 |
| 通讯作者 | she ym |
| 中文摘要 | matrix-assisted laser desorption ionization (maldi) mass spectrometry is difficult for the characterization of noncovalent complexes hitherto because of the limitations in acidic matrix, sample preparation, laser-induced polymerization and adduct formation with matrix. under our experimental conditions, sinapinic acid is used as a matrix, the specific noncovalent interactions of protein with fullerenols were observed by maldi mass spectrometry. some mass spectrometric features, such as mass shifts, broad adduct peaks and stoichiometries, showed that the specific non-covalent complexes between protein and fullerenols have been formed at a ratio of 1 : 4 for hemoglobin-fullerenols or 1 : 1 for myoglobin-fullerenols. the results implied that fullereneols could be used to protect partly hemoglobin from decomposition in acidic media, and therefore, it is possible to realize the molecular weight determination of a quaternary protein by maldi mass spectrometry via the addition of specific organic compound in the matrix. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000078261700009 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/22583]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | She YM,Ji YP,He YF,et al. Detection of specific noncovalent protein-fullerenols complexes by matrix-assisted laser desorption ionization mass spectrometry[J]. chemical journal of chinese universities-chinese,1998,19(11):1735-1738. |
| APA | She YM,Ji YP,He YF,&Liu SY.(1998).Detection of specific noncovalent protein-fullerenols complexes by matrix-assisted laser desorption ionization mass spectrometry.chemical journal of chinese universities-chinese,19(11),1735-1738. |
| MLA | She YM,et al."Detection of specific noncovalent protein-fullerenols complexes by matrix-assisted laser desorption ionization mass spectrometry".chemical journal of chinese universities-chinese 19.11(1998):1735-1738. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。